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CD300a antibody | P192

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Anti Human CD300a Antibody, clone P192 thumbnail image 1 Anti Human CD300a Antibody, clone P192 thumbnail image 2 Anti Human CD300a Antibody, clone P192 thumbnail image 3
Anti Human CD300a Antibody, clone P192 gallery image 1
Human peripheral blood monocytes stained with Mouse anti Human CD300a (MCA5747) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B).
Anti Human CD300a Antibody, clone P192 gallery image 2
Human peripheral blood monocytes stained with Mouse anti Human CD300a:FITC (MCA5747F).
Anti Human CD300a Antibody, clone P192 gallery image 3
Human peripheral blood monocytes stained with Mouse anti Human CD300a:RPE (MCA5747PE).

Mouse anti Human CD300a:RPE

Product Type
Monoclonal Antibody
Clone
P192
Isotype
IgG2a
Specificity
CD300a

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Product Code Applications Pack Size List Price Your Price Qty
MCA5747PE
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F 100 Tests loader
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Mouse anti Human CD300a antibody, clone P192 recognizes human CD300a, otherwise known as IRp60 or inhibitory receptor protein 60. CD300a is a ~60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.

Cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils significantly inhibits their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Polyclonal population of NK cells.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3U1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests
>
List Price Your Price
Description Mouse IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

Source Reference

  1. Cantoni, C. et al. (1999) Molecular and functional characterization of IRp60, a member of the immunoglobulin superfamily that functions as an inhibitory receptor in human NK cells.
    Eur J Immunol. 29: 3148-59.

References for CD300a antibody

  1. Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.
    J Immunol. 175: 7989-95.
  2. Bachelet, I. et al. (2006) Abrogation of allergic reactions by a bispecific antibody fragment linking IgE to CD300a.
    J Allergy Clin Immunol. 117: 1314-20.
  3. Bachelet, I. et al. (2008) Suppression of normal and malignant kit signaling by a bispecific antibody linking kit with CD300a.
    J Immunol. 180: 6064-9.
  4. Munitz, A. et al. (2006) The inhibitory receptor IRp60 (CD300a) suppresses the effects of IL-5, GM-CSF, and eotaxin on human peripheral blood eosinophils.
    Blood. 107:1996-2003.

Synonyms
IRp60
RRID
AB_11152762
UniProt
Q9UGN4
Entrez Gene
CD300A
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity

MCA5747PE

0311 1702

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