CD27 antibody | LT27

Mouse anti Human CD27:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
LT27
Isotype
IgG2a
Specificity
CD27

Product Code Applications Pack Size List Price Your Price Qty
MCA755A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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MCA755A647T
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F 25 Tests/0.25ml loader
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Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.

Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
Immunogen
Normal human blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG2a Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD27 antibody

  1. Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC
    in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411.
  2. Boxall, S. et al. (2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.
    Pediatr Res. 55 (3): 478-84.
  3. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
    Vet Immunol Immunopathol. 119: 115-22.
  4. Becker, P.D. et al. (2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ Mice
    PLoS One. 5(10). pii: e13137.
  5. van Zelm, M.C. et al. (2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
    J Exp Med. 204: 645-55.
  6. Steger, B. et al. (2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.
    Immunobiology. 219 (4): 247-60.

Flow Cytometry

RRID
AB_324802
UniProt
P26842
Entrez Gene
CD27
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0008588 release of cytoplasmic sequestered NF-kappaB
GO:0016064 immunoglobulin mediated immune response
GO:0043027 caspase inhibitor activity
GO:0045579 positive regulation of B cell differentiation
GO:0046330 positive regulation of JNK cascade
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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