Mouse anti Human CD25 antibody, clone BC96
recognizes the human CD25 cell surface antigen, also known as the Interleukin-2 receptor subunit alpha or TAC antigen.
CD25 is derived from a 272 amino acid precursor to yield a 251 amino acid monomeric receptor of ~53kDa which acts as the low affinity receptor for IL-2. CD25 can also form a heterodimer with CD122
to form the high affinity receptor for IL-2. CD25 is expressed by activated lymphocytes (Jackson et al. 1990
, Butcher et al. 1990
). Defects in CD4+/ CD25+ regulatory T cells have been implicated in the pathogenesis of autoimmune diabetes (Lindley et al. 2005
) and other autoimmune conditions (Sakaguchi et al. 1995
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to RPE-Cy5 - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|0.2%||Bovine Serum Albumin|
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
- Entrez Gene
- GO Terms
integral to membrane
interleukin-2 receptor activity
- Cy® and CyDye® are registered trademarks of GE Healthcare
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD25 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
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Useful Reagents Available
Product Specific References
References for CD25 antibody
Chapel, A. et al. (1992) Differential human immunodeficiency virus expression in CD4+ cloned lymphocytes: from viral latency to replication.
J Virol. 66 (6): 3966-70.
Ernst, C.W. et al. (2007) Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease.
Clin Exp Immunol. 148 (2): 271-80.
Barberá A et al. (2013) APL-1, an altered peptide ligand derived from human heat-shock protein 60, selectively induces apoptosis in activated CD4+ CD25+ T cells from peripheral blood of rheumatoid arthritis patients.
Int Immunopharmacol. 17 (4): 1075-83.
Duggleby, R.C. & Madrigal, J.A. (2014) Methods of detection of immune reconstitution and T regulatory cells by flow cytometry.
Methods Mol Biol. 1109: 159-86.