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CD21 antibody | Bu33

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Anti Human CD21 Antibody, clone Bu33 thumbnail image 1 Anti Human CD21 Antibody, clone Bu33 thumbnail image 2 Anti Human CD21 Antibody, clone Bu33 thumbnail image 3
Anti Human CD21 Antibody, clone Bu33 gallery image 1
Mouse anti Human CD21 antibody, clone Bu33 (MCA2499) used to stain formalin fixed, paraffin embedded human spleen sections by immunohistochemistry, visualized by biotinylated anti-Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue).
Anti Human CD21 Antibody, clone Bu33 gallery image 2
Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD19 (MCA1940SBY575) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD19 (MCA1940SBY575) and FITC conjugated Mouse anti Human CD21 (MCA2499F). All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD21 Antibody, clone Bu33 gallery image 3
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD19 (MCA1940SBB700) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD19 (MCA1940SBB700) and RPE conjugated Mouse anti Human CD21 (MCA2499PE). All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD21:FITC

Product Type
Monoclonal Antibody
Clone
Bu33
Isotype
IgG1
Specificity
CD21

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Product Code Applications Pack Size List Price Your Price Qty
MCA2499F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD21, clone Bu33 recognizes human CD21, a ~145 kDa cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol-3-kinase.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

References for CD21 antibody

  1. Ling, N.R. et al. (1998) Origin and properties of soluble CD21 (CR2) in human blood.
    Clin Exp Immunol. 113 (3): 360-6.
  2. Garland Publishing, Inc. (1998) Leucocyte Typing VI Garland Publishing, Inc.
    pp140-142
  3. Wei, F. et al. (2001) A man who inherited his SRY gene and Leri-Weill dyschondrosteosis from his mother and neurofibromatosis type 1 from his father.
    Am J Med Genet. 102 (4): 353-8.
  4. Mayall, S. et al. (2001) The anti-human CD21 antibody, Bu33, identifies equine B cells.
    J Comp Pathol. 124 (1): 83-7.
  5. Thornton, C.A. et al. (2002) Expression of CD21 and CD23 during human fetal development.
    Pediatr Res. 52 (2): 245-50.

Synonyms
CR2
UniProt
P20023
Entrez Gene
CR2
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004875 complement receptor activity
GO:0006958 complement activation, classical pathway
GO:0042803 protein homodimerization activity
GO:0045087 innate immune response

MCA2499F

165412

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