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CD195 antibody | T21/8

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Anti Human CD195 Antibody, clone T21/8 thumbnail image 1 Anti Human CD195 Antibody, clone T21/8 thumbnail image 2 Anti Human CD195 Antibody, clone T21/8 thumbnail image 3
Anti Human CD195 Antibody, clone T21/8 gallery image 1
Surface staining of human peripheral blood with Mouse anti Human CD195:APC (MCA6127APC)
Anti Human CD195 Antibody, clone T21/8 gallery image 2
Surface staining of human peripheral blood with Mouse anti Human CD195:RPE (MCA6127PE)
Anti Human CD195 Antibody, clone T21/8 gallery image 3
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse anti Human CD195 (MCA6127PE). All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD195:RPE

Product Type
Monoclonal Antibody
Clone
T21/8
Isotype
IgG1
Specificity
CD195

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Product Code Applications Pack Size List Price Your Price Qty
MCA6127PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
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Mouse anti Human CD195, clone T21/8 recognizes CD195 also known as C-C chemokine receptor 5 (CCR5) which belongs to the GPCR protein family and plays a role in inflammation by targeting cells to sites of inflammation.

CD195 is expressed in a range of hematopoietic cells including macrophages, dendritic cells and memory T cells. It is also expressed by non-hematopoietic cells including vascular smooth muscle cells and astrocytes (Barmania &Pepper 2013).

During inflammation CD195 expression is upregulated in CD8+ cells, allowing the cells to migrate to sites with CD4+ T cell and dendritic cell interactions (Castellino et al. 2006). An additional well characterized feature of CD195 is its role as an essential co-receptor for macrophage tropic HIV strains, allowing the virus to enter the target cell during initial infection (Dragic et al. 1996).

This clone T28/1 has been used in flow cytometry experiments to examine expression levels in CD195 overexpressing PM1 cells. These cells were then used to examine the effect of CD195 expression levels on HIV infections (Monde et al. 2007).

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
CCR5 peptide (Met1-Lys22) KLH conjugate
Purity
>95% by SDS PAGE
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

References for CD195 antibody

  1. Barmania, F. & Pepper, M.S. (2013) C-C chemokine receptor type five (CCR5): An emerging target for the control of HIV infection.
    Appl Transl Genom. 2: 3-16.
  2. Castellino, F. & Germain, R.N. (2006) Cooperation between CD4+ and CD8+ T cells: when, where, and how.
    Annu Rev Immunol. 24: 519-40.
  3. Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
    Nature. 381 (6584): 667-73.
  4. Monde, K. et al. (2007) Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5.
    J Biol Chem. 282 (51): 36923-32.

Synonyms
CCR5
UniProt
P51681
Entrez Gene
CCR5

MCA6127PE

149863 157293 164228 164833

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