CD195 antibody | T21/8
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|Mouse anti Human CD195, clone T21/8 recognizes CD195 also known as C-C chemokine receptor 5 (CCR5) which belongs to the GPCR protein family and plays a role in inflammation by targeting cells to sites of inflammation.
CD195 is expressed in a range of hematopoietic cells including macrophages, dendritic cells and memory T cells. It is also expressed by non-hematopoietic cells including vascular smooth muscle cells and astrocytes (Barmania &Pepper 2013).
During inflammation CD195 expression is upregulated in CD8+ cells, allowing the cells to migrate to sites with CD4+ T cell and dendritic cell interactions (Castellino et al. 2006). An additional well characterized feature of CD195 is its role as an essential co-receptor for macrophage tropic HIV strains, allowing the virus to enter the target cell during initial infection (Dragic et al. 1996).
This clone T28/1 has been used in flow cytometry experiments to examine expression levels in CD195 overexpressing PM1 cells. These cells were then used to examine the effect of CD195 expression levels on HIV infections (Monde et al. 2007).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- CCR5 peptide (Met1-Lys22) KLH conjugate
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting 1|
- 1For this target it is recommended that cell lysates are not boiled prior to SDS-PAGE
References for CD195 antibody
Barmania, F. & Pepper, M.S. (2013) C-C chemokine receptor type five (CCR5): An emerging target for the control of HIV infection.
Appl Transl Genom. 2: 3-16.
Castellino, F. & Germain, R.N. (2006) Cooperation between CD4+ and CD8+ T cells: when, where, and how.
Annu Rev Immunol. 24: 519-40.
Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
Nature. 381 (6584): 667-73.
Monde, K. et al. (2007) Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5.
J Biol Chem. 282 (51): 36923-32.
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