CD195 antibody | T21/8
Mouse anti Human CD195:APC
Mouse anti Human CD195
Mouse anti Human CD195:RPE
- Product Type
- Monoclonal Antibody
- Clone
- T21/8
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6127APC | F | 100 Tests/1ml |
|
|
| MCA6127 | E F IC IP WB* | 0.1 mg |
|
|
| MCA6127PE | F | 100 Tests/1ml |
|
Mouse anti Human CD195, clone T21/8 recognizes CD195 also known as C-C chemokine receptor 5 (CCR5) which belongs to the GPCR protein family and plays a role in inflammation by targeting cells to sites of inflammation.
CD195 is expressed in a range of hematopoietic cells including macrophages, dendritic cells and memory T cells. It is also expressed by non-hematopoietic cells including vascular smooth muscle cells and astrocytes (Barmania &Pepper 2013).
During inflammation CD195 expression is upregulated in CD8+ cells, allowing the cells to migrate to sites with CD4+ T cell and dendritic cell interactions (Castellino et al. 2006). An additional well characterized feature of CD195 is its role as an essential co-receptor for macrophage tropic HIV strains, allowing the virus to enter the target cell during initial infection (Dragic et al. 1996).
This clone T28/1 has been used in flow cytometry experiments to examine expression levels in CD195 overexpressing PM1 cells. These cells were then used to examine the effect of CD195 expression levels on HIV infections (Monde et al. 2007).
CD195 is expressed in a range of hematopoietic cells including macrophages, dendritic cells and memory T cells. It is also expressed by non-hematopoietic cells including vascular smooth muscle cells and astrocytes (Barmania &Pepper 2013).
During inflammation CD195 expression is upregulated in CD8+ cells, allowing the cells to migrate to sites with CD4+ T cell and dendritic cell interactions (Castellino et al. 2006). An additional well characterized feature of CD195 is its role as an essential co-receptor for macrophage tropic HIV strains, allowing the virus to enter the target cell during initial infection (Dragic et al. 1996).
This clone T28/1 has been used in flow cytometry experiments to examine expression levels in CD195 overexpressing PM1 cells. These cells were then used to examine the effect of CD195 expression levels on HIV infections (Monde et al. 2007).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- CCR5 peptide (Met1-Lys22) KLH conjugate
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of CD195 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| ELISA | |||
| Flow Cytometry | |||
| Immunocytochemistry | |||
| Immunoprecipitation | |||
| Western Blotting 1 | |||
| Flow Cytometry | Neat |
- 1For this target it is recommended that cell lysates are not boiled prior to SDS-PAGE
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
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