CD16 antibody | LNK16
Mouse anti Human CD16 antibody, clone LNK16 (MCA1193) used as a blocking agent to assess the effect of FcγRs on heparin binding protein (HBP) release by ELISA.
Image caption:
Antibodies against FcγRII inhibit HBP release.
Whole blood (diluted 1∶10) was incubated with antibodies against different FcγRs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.
From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F BA ResetMouse anti Human CD16:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- LNK16
- Isotype
- IgG1
- Specificity
- CD16
Antibody Quality and Performance Guarantee
| Mouse anti Human CD16 antibody, clone LNK16 recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Normal human peripheral blood granulocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for CD16 antibody
-
Tamm, A. & Schmidt, R.E. (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.
J Immunol. 157 (4): 1576-81. -
Fleit, H.B. et al. (1982) Human neutrophil Fc gamma receptor distribution and structure.
Proc Natl Acad Sci U S A. 79 (10): 3275-9. -
van de Winkel, J.G. & Capel, P.J. (1993) Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.
Immunol Today. 14 (5): 215-21. -
Pillay, J. et al. (2012) A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1.
J Clin Invest. 122: 327-36. -
Morris, D.L. et al. (2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.
Eur J Hum Genet. 18 (9): 1027-31. -
Kamp, V.M. et al. (2012) Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion.
J Leukoc Biol. 92 (5): 1011-20. -
Hanna, J. et al. (2003) CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.
Blood. 102:1569-77. -
Moalli, F. et al. (2010) Role of complement and Fc{gamma} receptors in the protective activity of the long pentraxin PTX3 against Aspergillus fumigatus.
Blood. 116: 5170-80.
View The Latest Product References
-
Armour, K.L. et al. (2010) Expression of human FcgammaRIIIa as a GPI-linked molecule on CHO cells to enable measurement of human IgG binding.
J Immunol Methods. 354: 20-33. -
Welters, I.D. et al. (2010) Ketamine inhibits transcription factors activator protein 1 and nuclear factor-kappaB, interleukin-8 production, as well as CD11b and CD16 expression: studies in human leukocytes and leukocytic cell lines.
Anesth Analg. 110: 934-41. -
Bowles, J.A. et al. (2006) Anti-CD20 monoclonal antibody with enhanced affinity for CD16 activates NK cells at lower concentrations and more effectively than rituximab.
Blood. 108: 2648-54. -
Meknache, N. et al. (2009) Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B).
J Immunol. 182: 2542-50. -
Lee, S.F. et al. (1999) Cytokine receptor common beta chain as a potential activator of cytokine withdrawal-induced apoptosis.
Mol Cell Biol. 19: 7399-409. -
Sironi, M. et al. (2006) Differential regulation of chemokine production by Fcgamma receptor engagement in human monocytes: association of CCL1 with a distinct form of M2 monocyte activation (M2b, Type 2).
J Leukoc Biol. 80: 342-9. -
Weissmüller, S. et al. (2012) ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Blood. 119: 6268-77. -
Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4: e1000149. -
Onodera, R. et al. (2017) Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay.
Transfusion. 57 (11): 2586-94.
Further Reading
-
Ravetch, J.V. & Kinet, J.P. (1991) Fc receptors.
Annu Rev Immunol. 9: 457-92.
- Synonyms
- FcRIII
- RRID
- AB_324855
- UniProt
- P08637
- O75015
- Entrez Gene
- FCGR3B
- FCGR3A
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0031225 anchored to membrane
- GO:0019864 IgG binding
- GO:0016021 integral to membrane
- GO:0050776 regulation of immune response
MCA1193A647
1607
MCA1193A647T
0115
148705
158342
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