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CD16 antibody | LNK16

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Anti Human CD16 Antibody, clone LNK16 thumbnail image 1 Anti Human CD16 Antibody, clone LNK16 thumbnail image 2 Anti Human CD16 Antibody, clone LNK16 thumbnail image 3 Anti Human CD16 Antibody, clone LNK16 thumbnail image 4
Anti Human CD16 Antibody, clone LNK16 gallery image 1
Figure A. RPE conjugated mouse anti human CD3 (MCA463PE ) and Alexa®647 conjugated Mouse IgG1 isotype control (MCA928A647 ). Figure B. RPE conjugated mouse anti human CD3 (MCA463PE ) and Alexa®647 conjugated mouse anti human CD16 (MCA1193A647 ). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A ).
Anti Human CD16 Antibody, clone LNK16 gallery image 2
Figure A. Alexa®488 conjugated mouse anti human CD56 (MCA2693A488 ) and Alexa®647 conjugated Mouse IgG1 isotype control (MCA928A647 ). Figure B. Alexa®488 conjugated mouse anti human CD56 (MCA2693A488 ) and Alexa®647 conjugated mouse anti human CD16 (MCA1193A647 ). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A ).
Anti Human CD16 Antibody, clone LNK16 gallery image 3
Published customer image:
Mouse anti Human CD16 antibody, clone LNK16 (MCA1193) used as a blocking agent to assess the effect of FcγRs on heparin binding protein (HBP) release by ELISA.
Image caption:
Antibodies against FcγRII inhibit HBP release.
Whole blood (diluted 1∶10) was incubated with antibodies against different FcγRs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.

From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD16 Antibody, clone LNK16 gallery image 4
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD56 (MCA2693A488) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD56 (MCA2693A700) and Amethyst Orange conjugated Mouse anti Human CD16 (MCA1193AMO). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human CD16:Alexa Fluor® 647

Mouse anti Human CD16:Biotin

Mouse anti Human CD16:FITC

Mouse anti Human CD16:Low Endotoxin

Mouse anti Human CD16

Mouse anti Human CD16:Amethyst Orange

Product Type
Monoclonal Antibody
Clone
LNK16
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1193A647T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA1193A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1193B
Datasheet
F
MSDS
0.1 mg loader

MCA1193FT
Datasheet
F
MSDS
25 µg loader

MCA1193F
Datasheet
F
MSDS
0.1 mg loader

MCA1193EL
Datasheet
F FN IP
MSDS
0.5 mg loader

MCA1193T
Datasheet
F IP
MSDS
25 µg loader

MCA1193GA
Datasheet
F IP
MSDS
0.1 mg loader

MCA1193
Datasheet
F IP
MSDS
0.2 mg loader

MCA1193AMO
Datasheet
F
MSDS
0.1 mg loader

Mouse anti Human CD16 antibody, clone LNK16 recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.

CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Normal human peripheral blood granulocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P08637
O75015
Entrez Gene
FCGR3B
FCGR3A
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0050776 regulation of immune response
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/5
Flow Cytometry Neat
Flow Cytometry 1/100 1/200
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control:Low Endotoxin MCA928EL C F 0.5 mg loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD16 antibody

  1. Tamm, A. & Schmidt, R.E. (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.
    J Immunol. 157 (4): 1576-81.
  2. Fleit, H.B. et al. (1982) Human neutrophil Fc gamma receptor distribution and structure.
    Proc Natl Acad Sci U S A. 79 (10): 3275-9.
  3. van de Winkel, J.G. & Capel, P.J. (1993) Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.
    Immunol Today. 14 (5): 215-21.
  4. Pillay, J. et al. (2012) A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1.
    J Clin Invest. 122: 327-36.
  5. Morris, D.L. et al. (2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.
    Eur J Hum Genet. 18 (9): 1027-31.
  6. Kamp, V.M. et al. (2012) Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion.
    J Leukoc Biol. 92 (5): 1011-20.
  7. Hanna, J. et al. (2003) CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.
    Blood. 102:1569-77.
  8. Moalli, F. et al. (2010) Role of complement and Fc{gamma} receptors in the protective activity of the long pentraxin PTX3 against Aspergillus fumigatus.
    Blood. 116: 5170-80.
  9. Armour, K.L. et al. (2010) Expression of human FcgammaRIIIa as a GPI-linked molecule on CHO cells to enable measurement of human IgG binding.
    J Immunol Methods. 354: 20-33.
  10. Welters, I.D. et al. (2010) Ketamine inhibits transcription factors activator protein 1 and nuclear factor-kappaB, interleukin-8 production, as well as CD11b and CD16 expression: studies in human leukocytes and leukocytic cell lines.
    Anesth Analg. 110: 934-41.
  11. Bowles, J.A. et al. (2006) Anti-CD20 monoclonal antibody with enhanced affinity for CD16 activates NK cells at lower concentrations and more effectively than rituximab.
    Blood. 108: 2648-54.
  12. Meknache, N. et al. (2009) Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B).
    J Immunol. 182: 2542-50.
  13. Lee, S.F. et al. (1999) Cytokine receptor common beta chain as a potential activator of cytokine withdrawal-induced apoptosis.
    Mol Cell Biol. 19: 7399-409.
  14. Sironi, M. et al. (2006) Differential regulation of chemokine production by Fcgamma receptor engagement in human monocytes: association of CCL1 with a distinct form of M2 monocyte activation (M2b, Type 2).
    J Leukoc Biol. 80: 342-9.
  15. Weissmüller, S. et al. (2012) ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood. 119: 6268-77.
  16. Kahn, F. et al. (2008) Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response.
    PLoS Pathog. 4: e1000149.
  17. Onodera, R. et al. (2017) Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay.
    Transfusion. 57 (11): 2586-94.

Further Reading

  1. Ravetch, J.V. & Kinet, J.P. (1991) Fc receptors.
    Annu Rev Immunol. 9: 457-92.
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