CD154 antibody | YMF323.6.2

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Rat anti Human CD154:RPE

Product Type
Monoclonal Antibody
Clone
YMF323.6.2
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1938PE F 100 Tests
Rat anti Human CD154 antibody, clone YMF323.6.2 recognizes the human CD154 cell surface antigen, a 32kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells. Rat anti Human CD154 antibody, clone YMF323.6.2 also recognizes the soluble form of CD154.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P29965 Related reagents
Entrez Gene
CD40LG Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0030168 platelet activation
GO:0032735 positive regulation of interleukin-12 production
GO:0042100 B cell proliferation
GO:0045190 isotype switching
Regulatory
For research purposes only

Applications of CD154 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:RPE MCA6005PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F