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CD154 antibody | YMF323.6.2

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Anti Human CD154 Antibody, clone YMF323.6.2 thumbnail image 1 Anti Human CD154 Antibody, clone YMF323.6.2 thumbnail image 2
Anti Human CD154 Antibody, clone YMF323.6.2 gallery image 1
Figure A. Unstimulated cells stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and PE conjugated Mouse anti Human CD154 (MCA1938PE). Figure B. Cells stimulated with PMA and ionomycin for 5 hr then stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and PE conjugated Mouse anti Human CD154 (MCA1938PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD154 Antibody, clone YMF323.6.2 gallery image 2
Recombinant Human CD154 (PHP126) detected with Rat anti Human CD154 (MCA1938)

Rat anti Human CD154:RPE

Product Type
Monoclonal Antibody
Clone
YMF323.6.2
Isotype
IgG2a
Specificity
CD154

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
MCA1938PE
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Rat anti Human CD154 antibody, clone YMF323.6.2 recognizes the human CD154 cell surface antigen, a 32kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells. Rat anti Human CD154 antibody, clone YMF323.6.2 also recognizes the soluble form of CD154.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

Synonyms
CD40 Ligand
TRAP
UniProt
P29965
Entrez Gene
CD40LG
GO Terms
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0030168 platelet activation
GO:0032735 positive regulation of interleukin-12 production
GO:0042100 B cell proliferation
GO:0045190 isotype switching
View more products with CD154 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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