CD154 antibody | MK13A4
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Mouse anti Human CD154 antibody, clone MK13A4 recognizes the human CD154 cell surface antigen, a 32 kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells.
Mouse anti Human CD154 antibody, clone MK13A4 has been reported to block binding of CD40 ligand to its receptor CD40.
Mouse anti Human CD154 antibody, clone MK13A4 has been reported to block binding of CD40 ligand to its receptor CD40.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Tris buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at -20oC.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P29965
- Entrez Gene
- CD40LG
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005174 CD40 receptor binding
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- GO:0030168 platelet activation
- GO:0032735 positive regulation of interleukin-12 production
- GO:0042100 B cell proliferation
- GO:0045190 isotype switching
- Regulatory
- For research purposes only
Applications of CD154 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Functional Assays | |||
| Flow Cytometry | Neat | 1/20 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD154 antibody
-
Koumas, L. et al. (2001) Fibroblast heterogeneity: existence of functionally distinct Thy 1(+) and Thy 1(-) human female reproductive tract fibroblasts.
Am J Pathol. 159 (3): 925-35. -
Kaufman, J. et al. (2004) Expression of CD154 (CD40 ligand) by human lung fibroblasts: differential regulation by IFN-gamma and IL-13, and implications for fibrosis.
J Immunol. 172:1862-71 -
Arita, S. et al. (2008) B cell activation regulates exosomal HLA production.
Eur J Immunol. 38 (5): 1423-34. -
Deambrosis, I. et al. (2009) Inhibition of CD40-CD154 costimulatory pathway by a cyclic peptide targeting CD154.
J Mol Med (Berl). 87 (2): 181-97.
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