CD154 antibody | MK13A4

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Mouse anti Human CD154:Preservative Free

Mouse anti Human CD154:FITC

Product Type
Monoclonal Antibody
Clone
MK13A4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1968Z F FN 0.1 mg
MCA1968F F 100 Tests
Mouse anti Human CD154 antibody, clone MK13A4 recognizes the human CD154 cell surface antigen, a 32 kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells.

Mouse anti Human CD154 antibody, clone MK13A4 has been reported to block binding of CD40 ligand to its receptor CD40.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Tris buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
0.02%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at -20oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P29965 Related reagents
Entrez Gene
CD40LG Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0030168 platelet activation
GO:0032735 positive regulation of interleukin-12 production
GO:0042100 B cell proliferation
GO:0045190 isotype switching
Regulatory
For research purposes only

Applications of CD154 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Flow Cytometry Neat 1/20
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD154 antibody

  1. Koumas, L. et al. (2001) Fibroblast heterogeneity: existence of functionally distinct Thy 1(+) and Thy 1(-) human female reproductive tract fibroblasts.
    Am J Pathol. 159 (3): 925-35.
  2. Kaufman, J. et al. (2004) Expression of CD154 (CD40 ligand) by human lung fibroblasts: differential regulation by IFN-gamma and IL-13, and implications for fibrosis.
    J Immunol. 172:1862-71
  3. Arita, S. et al. (2008) B cell activation regulates exosomal HLA production.
    Eur J Immunol. 38 (5): 1423-34.
  4. Deambrosis, I. et al. (2009) Inhibition of CD40-CD154 costimulatory pathway by a cyclic peptide targeting CD154.
    J Mol Med (Berl). 87 (2): 181-97.