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CD154 antibody | MK13A4

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Mouse anti Human CD154:FITC

Product Type
Monoclonal Antibody
Clone
MK13A4
Isotype
IgG1
Specificity
CD154

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MCA1968F
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Mouse anti Human CD154 antibody, clone MK13A4 recognizes the human CD154 cell surface antigen, a 32 kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells.

Mouse anti Human CD154 antibody, clone MK13A4 has been reported to block binding of CD40 ligand to its receptor CD40.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Tris buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
1% bovine serum albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD154 antibody

  1. Kaufman, J. et al. (2004) Expression of CD154 (CD40 ligand) by human lung fibroblasts: differential regulation by IFN-gamma and IL-13, and implications for fibrosis.
    J Immunol. 172:1862-71
  2. Arita, S. et al. (2008) B cell activation regulates exosomal HLA production.
    Eur J Immunol. 38 (5): 1423-34.
  3. Deambrosis, I. et al. (2009) Inhibition of CD40-CD154 costimulatory pathway by a cyclic peptide targeting CD154.
    J Mol Med (Berl). 87 (2): 181-97.

Synonyms
CD40 Ligand
TRAP
RRID
AB_323214
UniProt
P29965
Entrez Gene
CD40LG
GO Terms
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0030168 platelet activation
GO:0032735 positive regulation of interleukin-12 production
GO:0042100 B cell proliferation
GO:0045190 isotype switching

MCA1968F

130515 160629

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