CD150 antibody | SLAM.4
Mouse anti Human CD150:RPE
- Product Type
- Monoclonal Antibody
- Clone
- SLAM.4
- Isotype
- IgG1
- Specificity
- CD150
Antibody Quality and Performance Guarantee
| Mouse anti Human CD150 antibody, clone SLAM.4 recognizes CD150 also known as signaling lymphocyte activation molecule (SLAM), is a member of the immunoglobulin superfamily. CD150 is expressed in dendritic cells, Natural Killer (NK) cells, B and T cells (Bhattocharjee and Yadava 2018). CD150 is a membrane phosphoglycoprotein which contains 2 extracellular domains corresponding to a variable and constant region, a general feature of the Ig superfamily (Cocks et al. 1995). CD150 also has a cytoplasmic domain which contains 3 unique tyrosine motifs with a conserved TxYxxV sequence. This tyrosine motif is the binding domain for SLAM-associated protein (SAP) which form a complex together (Sayos et al 1998, Poy et al. 1999). This complex plays a role in the regulation of Natural killer (NK) and T cell development. Mutations to SAP in humans results in X-linked lymphoproliferative disease which is a fatal immunological disorder (Coffey et al. 1998). Activation of CD150 co-activates T- or B-lymphocytes responses and plays a role in bidirectional T-B cell stimulation (Carballido et al. 1997). It can also regulate NK and T cell development, B cell regulation and antibody production/isotype switching (Veillette 2006). CD150 is also the receptor that the measles virus recognizes (Tatsuo et al. 2000). The SLAM.4 clone has been used in flow cytometry to analyze the cell distribution of CD150 on leucocytes and lymphocytes as well as to distinguish between subsets of CD4+ and CD8+ T lymphocytes (Romero et al. 2001). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Human CD150-transfected 300.19 cells
- Purity
- >95% by SDS PAGE
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG1 Negative Control:RPE | ||||||
References for CD150 antibody
-
Bhattacharjee, S. & Yadava, P.K. (2018) Measles virus: Background and oncolytic virotherapy.
Biochem Biophys Rep. 13: 58-62. -
Cocks, B.G. et al. (1995) A novel receptor involved in T-cell activation.
Nature. 376 (6537): 260-3. -
Sayos, J. et al. (1998) The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
Nature. 395 (6701): 462-9. -
Poy, F. et al. (1999) Crystal structures of the XLP protein SAP reveal a class of SH2 domains with extended, phosphotyrosine-independent sequence recognition.
Mol Cell. 4 (4): 555-61. -
Coffey, A.J. et al. (1998) Host response to EBV infection in X-linked lymphoproliferative disease results from mutations in an SH2-domain encoding gene.
Nat Genet. 20 (2): 129-35. -
Carballido, J.M. et al. (1997) The intensity of T cell receptor engagement determines the cytokine pattern of human allergen-specific T helper cells.
Eur J Immunol. 27 (2): 515-21. -
Veillette, A. (2006) NK cell regulation by SLAM family receptors and SAP-related adapters.
Immunol Rev. 214: 22-34. -
Tatsuo, H. et al. (2000) SLAM (CDw150) is a cellular receptor for measles virus.
Nature. 406 (6798): 893-7.
View The Latest Product References
- Synonyms
- SLAM
- UniProt
- Q13291
- Entrez Gene
- SLAMF1
- GO Terms
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0008284 positive regulation of cell proliferation
- GO:0044419 interspecies interaction between organisms
- GO:0046649 lymphocyte activation
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