CD150 antibody | SLAM.4
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|Mouse anti Human CD150 antibody, clone SLAM.4 recognizes CD150 also known as signaling lymphocyte activation molecule (SLAM), is a member of the immunoglobulin superfamily. CD150 is expressed in dendritic cells, Natural Killer (NK) cells, B and T cells (Bhattocharjee and Yadava 2018).
CD150 is a membrane phosphoglycoprotein which contains 2 extracellular domains corresponding to a variable and constant region, a general feature of the Ig superfamily (Cocks et al. 1995). CD150 also has a cytoplasmic domain which contains 3 unique tyrosine motifs with a conserved TxYxxV sequence. This tyrosine motif is the binding domain for SLAM-associated protein (SAP) which form a complex together (Sayos et al 1998, Poy et al. 1999). This complex plays a role in the regulation of Natural killer (NK) and T cell development. Mutations to SAP in humans results in X-linked lymphoproliferative disease which is a fatal immunological disorder (Coffey et al. 1998). Activation of CD150 co-activates T- or B-lymphocytes responses and plays a role in bidirectional T-B cell stimulation (Carballido et al. 1997). It can also regulate NK and T cell development, B cell regulation and antibody production/isotype switching (Veillette 2006). CD150 is also the receptor that the measles virus recognizes (Tatsuo et al. 2000).
The SLAM.4 clone has been used in flow cytometry to analyze the cell distribution of CD150 on leucocytes and lymphocytes as well as to distinguish between subsets of CD4+ and CD8+ T lymphocytes (Romero et al. 2001).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant and 0.2 μm filter sterilized
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Human CD150-transfected 300.19 cells
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
References for CD150 antibody
Bhattacharjee, S. & Yadava, P.K. (2018) Measles virus: Background and oncolytic virotherapy.
Biochem Biophys Rep. 13: 58-62.
Cocks, B.G. et al. (1995) A novel receptor involved in T-cell activation.
Nature. 376 (6537): 260-3.
Sayos, J. et al. (1998) The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
Nature. 395 (6701): 462-9.
Poy, F. et al. (1999) Crystal structures of the XLP protein SAP reveal a class of SH2 domains with extended, phosphotyrosine-independent sequence recognition.
Mol Cell. 4 (4): 555-61.
Coffey, A.J. et al. (1998) Host response to EBV infection in X-linked lymphoproliferative disease results from mutations in an SH2-domain encoding gene.
Nat Genet. 20 (2): 129-35.
Carballido, J.M. et al. (1997) The intensity of T cell receptor engagement determines the cytokine pattern of human allergen-specific T helper cells.
Eur J Immunol. 27 (2): 515-21.
Veillette, A. (2006) NK cell regulation by SLAM family receptors and SAP-related adapters.
Immunol Rev. 214: 22-34.
Tatsuo, H. et al. (2000) SLAM (CDw150) is a cellular receptor for measles virus.
Nature. 406 (6798): 893-7.
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