CD150 antibody | SLAM.4
Mouse anti Human CD150 antibody, clone SLAM.4 recognizes CD150 also known as signaling lymphocyte activation molecule (SLAM), is a member of the immunoglobulin superfamily. CD150 is expressed in dendritic cells, Natural Killer (NK) cells, B and T cells (Bhattocharjee and Yadava 2018).
CD150 is a membrane phosphoglycoprotein which contains 2 extracellular domains corresponding to a variable and constant region, a general feature of the Ig superfamily (Cocks et al. 1995). CD150 also has a cytoplasmic domain which contains 3 unique tyrosine motifs with a conserved TxYxxV sequence. This tyrosine motif is the binding domain for SLAM-associated protein (SAP) which form a complex together (Sayos et al 1998, Poy et al. 1999). This complex plays a role in the regulation of Natural killer (NK) and T cell development. Mutations to SAP in humans results in X-linked lymphoproliferative disease which is a fatal immunological disorder (Coffey et al. 1998). Activation of CD150 co-activates T- or B-lymphocytes responses and plays a role in bidirectional T-B cell stimulation (Carballido et al. 1997). It can also regulate NK and T cell development, B cell regulation and antibody production/isotype switching (Veillette 2006). CD150 is also the receptor that the measles virus recognizes (Tatsuo et al. 2000).
The SLAM.4 clone has been used in flow cytometry to analyze the cell distribution of CD150 on leucocytes and lymphocytes as well as to distinguish between subsets of CD4+ and CD8+ T lymphocytes (Romero et al. 2001).
CD150 is a membrane phosphoglycoprotein which contains 2 extracellular domains corresponding to a variable and constant region, a general feature of the Ig superfamily (Cocks et al. 1995). CD150 also has a cytoplasmic domain which contains 3 unique tyrosine motifs with a conserved TxYxxV sequence. This tyrosine motif is the binding domain for SLAM-associated protein (SAP) which form a complex together (Sayos et al 1998, Poy et al. 1999). This complex plays a role in the regulation of Natural killer (NK) and T cell development. Mutations to SAP in humans results in X-linked lymphoproliferative disease which is a fatal immunological disorder (Coffey et al. 1998). Activation of CD150 co-activates T- or B-lymphocytes responses and plays a role in bidirectional T-B cell stimulation (Carballido et al. 1997). It can also regulate NK and T cell development, B cell regulation and antibody production/isotype switching (Veillette 2006). CD150 is also the receptor that the measles virus recognizes (Tatsuo et al. 2000).
The SLAM.4 clone has been used in flow cytometry to analyze the cell distribution of CD150 on leucocytes and lymphocytes as well as to distinguish between subsets of CD4+ and CD8+ T lymphocytes (Romero et al. 2001).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant and 0.2 μm filter sterilized
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Human CD150-transfected 300.19 cells
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13291
- Entrez Gene
- SLAMF1
- GO Terms
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0008284 positive regulation of cell proliferation
- GO:0044419 interspecies interaction between organisms
- GO:0046649 lymphocyte activation
- Regulatory
- For research purposes only
Applications of CD150 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunocytochemistry | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
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| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Product Specific References
References for CD150 antibody
-
Bhattacharjee, S. & Yadava, P.K. (2018) Measles virus: Background and oncolytic virotherapy.
Biochem Biophys Rep. 13: 58-62. -
Cocks, B.G. et al. (1995) A novel receptor involved in T-cell activation.
Nature. 376 (6537): 260-3. -
Sayos, J. et al. (1998) The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
Nature. 395 (6701): 462-9. -
Poy, F. et al. (1999) Crystal structures of the XLP protein SAP reveal a class of SH2 domains with extended, phosphotyrosine-independent sequence recognition.
Mol Cell. 4 (4): 555-61. -
Coffey, A.J. et al. (1998) Host response to EBV infection in X-linked lymphoproliferative disease results from mutations in an SH2-domain encoding gene.
Nat Genet. 20 (2): 129-35. -
Carballido, J.M. et al. (1997) The intensity of T cell receptor engagement determines the cytokine pattern of human allergen-specific T helper cells.
Eur J Immunol. 27 (2): 515-21. -
Veillette, A. (2006) NK cell regulation by SLAM family receptors and SAP-related adapters.
Immunol Rev. 214: 22-34. -
Tatsuo, H. et al. (2000) SLAM (CDw150) is a cellular receptor for measles virus.
Nature. 406 (6798): 893-7.
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