CD150 antibody | SLAM.4

Mouse anti Human CD150:Preservative Free

Product Type
Monoclonal Antibody
Clone
SLAM.4
Isotype
IgG1
Specificity
CD150

Product Code Applications Pack Size List Price Your Price Qty
MCA6121Z
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SDS Safety Datasheet SDS
F IC IP 0.1 mg loader
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loader
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Mouse anti Human CD150 antibody, clone SLAM.4 recognizes CD150 also known as signaling lymphocyte activation molecule (SLAM), is a member of the immunoglobulin superfamily. CD150 is expressed in dendritic cells, Natural Killer (NK) cells, B and T cells (Bhattocharjee and Yadava 2018).

CD150 is a membrane phosphoglycoprotein which contains 2 extracellular domains corresponding to a variable and constant region, a general feature of the Ig superfamily (Cocks et al. 1995). CD150 also has a cytoplasmic domain which contains 3 unique tyrosine motifs with a conserved TxYxxV sequence. This tyrosine motif is the binding domain for SLAM-associated protein (SAP) which form a complex together (Sayos et al 1998, Poy et al. 1999). This complex plays a role in the regulation of Natural killer (NK) and T cell development. Mutations to SAP in humans results in X-linked lymphoproliferative disease which is a fatal immunological disorder (Coffey et al. 1998). Activation of CD150 co-activates T- or B-lymphocytes responses and plays a role in bidirectional T-B cell stimulation (Carballido et al. 1997). It can also regulate NK and T cell development, B cell regulation and antibody production/isotype switching (Veillette 2006). CD150 is also the receptor that the measles virus recognizes (Tatsuo et al. 2000).

The SLAM.4 clone has been used in flow cytometry to analyze the cell distribution of CD150 on leucocytes and lymphocytes as well as to distinguish between subsets of CD4+ and CD8+ T lymphocytes (Romero et al. 2001).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant and 0.2 μm filter sterilized
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Human CD150-transfected 300.19 cells
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunocytochemistry
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD150 antibody

  1. Bhattacharjee, S. & Yadava, P.K. (2018) Measles virus: Background and oncolytic virotherapy.
    Biochem Biophys Rep. 13: 58-62.
  2. Cocks, B.G. et al. (1995) A novel receptor involved in T-cell activation.
    Nature. 376 (6537): 260-3.
  3. Sayos, J. et al. (1998) The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
    Nature. 395 (6701): 462-9.
  4. Poy, F. et al. (1999) Crystal structures of the XLP protein SAP reveal a class of SH2 domains with extended, phosphotyrosine-independent sequence recognition.
    Mol Cell. 4 (4): 555-61.
  5. Coffey, A.J. et al. (1998) Host response to EBV infection in X-linked lymphoproliferative disease results from mutations in an SH2-domain encoding gene.
    Nat Genet. 20 (2): 129-35.
  6. Carballido, J.M. et al. (1997) The intensity of T cell receptor engagement determines the cytokine pattern of human allergen-specific T helper cells.
    Eur J Immunol. 27 (2): 515-21.
  7. Veillette, A. (2006) NK cell regulation by SLAM family receptors and SAP-related adapters.
    Immunol Rev. 214: 22-34.
  8. Tatsuo, H. et al. (2000) SLAM (CDw150) is a cellular receptor for measles virus.
    Nature. 406 (6798): 893-7.
  9. View The Latest Product References

Synonyms
SLAM
UniProt
Q13291
Entrez Gene
SLAMF1
GO Terms
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0008284 positive regulation of cell proliferation
GO:0044419 interspecies interaction between organisms
GO:0046649 lymphocyte activation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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