CD144 antibody | 55-7H1
Mouse anti Human CD144 antibody, clone 55-7H1 (MCA6119) used to identify VE-Cadherin expression on endothelial cells by immunofluorescence.
Image caption:
Viability, confluence and quantification of ECs adherent to HFM. Viable ECs on AH (A) and FN (B) coated HFM were visualized using calcein staining (green); Scale: 200 μm, VE-cadherin (green) and Hoechst (blue) indicated intercellular connection between ECs on AH (C) and FN (D) coated HFM; Scale: 100 μm. (E) Quantification of adherent ECs on AH and FN coated HFM, ECs on non-coated (NC) HFM were not detectable (N.D.).
From: Pflaum M, Jurmann S, Katsirntaki K, Mälzer M, Haverich A, Wiegmann B.
Towards Biohybrid Lung Development—Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization.
Membranes. 2022; 12(1):35.
doi: 10.3390/membranes12010035
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD144 antibody, clone 55-7H1 (MCA6119) used to stain endothelial cells in vitro by immunofluorescence.
Image caption:
Confluent EML on HFMs remained unaffected after medium exchange during 24 h rotational seeding.CLSM images for the detection of VE-cadherin (magenta) on HFM samples cultivated without (E) or with MExch (F). Nuclei were counterstained with Hoechst 33342 (blue). Scale: 200 μm.
From: Alabdullh, H.A.; Pflaum, M.; Mälzer, M.; Kipp, M.; Naghilouy-Hidaji, H.; Adam, D.; Kühn, C.; Natanov, R.; Niehaus, A.; Haverich, A.; Wiegmann, B.
Biohybrid Lung Development: Towards Complete Endothelialization of an Assembled Extracorporeal Membrane Oxygenator.
Bioengineering 2023, 10, 72.
doi: 10.3390/bioengineering10010072
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD144:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 55-7H1
- Isotype
- IgG1
- Specificity
- CD144
Antibody Quality and Performance Guarantee
| Mouse anti Human CD144, clone 55-7H1 recognizes CD144, also known as cadherin 5, VE-cadherin or 7B4 antigen. CD144 is a 737 amino acid, ~130 kDa single pass type 1 transmembrane glycoprotein involved in cellular adhesion processes and is expressed by endothelial cells. CD 144 has a 25 amino acid signal peptide and a 22 amino acid pro-peptide region. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Human endothelial cells
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG1 Negative Control:RPE | ||||||
References for CD144 antibody
-
Pflaum, M. et al. (2021) Towards Biohybrid Lung Development-Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization.
Membranes (Basel). 12 (1): 35. -
Alabdullh, A.H. et al. (2023) Biohybrid Lung Development: Towards Complete Endothelialization of an Assembled Extracorporeal Membrane Oxygenator
Bioengineering. 10 (1): 72.
- Synonyms
- VE-CADHERIN
- UniProt
- P33151
- Entrez Gene
- CDH5
- GO Terms
- GO:0005509 calcium ion binding
- GO:0005102 receptor binding
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0007156 homophilic cell adhesion
- GO:0008013 beta-catenin binding
- GO:0034329 cell junction assembly
- GO:0034332 adherens junction organization
- GO:0044325 ion channel binding
- View More GO Terms
MCA6119PE
149844
158392
166399
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