CD14 antibody | UCHM1
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Mouse anti Human CD14:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD14 antibody, clone UCHM1 recognizes a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative (Hogg et al. 1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey Fish Trout
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Human Thymocytes followed by peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519
- For research purposes only
- 12 months from date of despatch
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or firstname.lastname@example.org
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.
Immunology. 53 (4): 753-67.
References for CD14 antibody
Linch, D.C. et al. (1984) Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.
Blood. 63 (3): 566-73.
Hogg, N. & Horton, M.A. (1987) Myeloid antigens: new and previously defined clusters
in Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J.,et al.
Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.
Leucocyte Typing IV. Oxford University Press p 1058-63.
Hsu, T.L. et al. (2002) Modulation of dendritic cell differentiation and maturation by decoy receptor 3.
J Immunol. 168: 4846-53.
Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.
Infect Immun. 70: 6688-96.
Kämmerer, U. et al. (2003) Unique appearance of proliferating antigen-presenting cells expressing DC-SIGN (CD209) in the decidua of early human pregnancy.
Am J Pathol. 162: 887-96.
Köller, M. et al. (2004) Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients.
Int Immunol. 16: 1595-604.
Goddard, S. et al. (2004) Interleukin-10 secretion differentiates dendritic cells from human liver and skin.
Am J Pathol. 164: 511-9.
View The Latest Product References
Chang, Y.C. et al. (2004) Modulation of macrophage differentiation and activation by decoy receptor 3.
J Leukoc Biol. 75: 486-94.
Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
Blood. 106: 3567-74.
Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
J Immunol. 176 (10): 5730-4.
Fischer, U. and Koellner, B. (2007) Cross-reactivity of human leukocyte differentiation antigen monoclonal antibodies on carp and rainbow trout cells.
Vet Immunol Immunopathol. 119: 142-55.
Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status.
Arterioscler Thromb Vasc Biol. 28: 1491-8.
Iking-Konert, C. et al. (2008) T lymphocytes in patients with primary vasculitis: expansion of CD8+ T cells with the propensity to activate polymorphonuclear neutrophils.
Rheumatology (Oxford). 47: 609-16.
Angel, C.E. et al. (2009) Distinctive localization of antigen-presenting cells in human lymph nodes.
Blood. 113: 1257-67.
Brook, F.A. et al. (2010) Derivation and characterisation of the human embryonic stem cell line, OxF1.
In Vitro Cell Dev Biol Anim. 46: 173-7.
Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12:2.
Din JN et al. (2013) Effect of ω-3 fatty acid supplementation on endothelial function, endogenous fibrinolysis and platelet activation in male cigarette smokers.
Heart. 99 (3): 168-74.
Bromberek, J.L. et al. (2016) Breed Distribution and Clinical Characteristics of B Cell Chronic Lymphocytic Leukemia in Dogs.
J Vet Intern Med. 30 (1): 215-22.
Spiller, K.L. et al. (2016) Differential gene expression in human, murine, and cell line-derived macrophages upon polarization.
Exp Cell Res. 347 (1): 1-13.
Kannegieter, N.M. et al. (2018) Analysis of NFATc1 amplification in T cells for pharmacodynamic monitoring of tacrolimus in kidney transplant recipients.
PLoS One. 13 (7): e0201113.
Wu, T.C. et al. (2018) IL1 Receptor Antagonist Controls Transcriptional Signature of Inflammation in Patients with Metastatic Breast Cancer.
Cancer Res. 78 (18): 5243-58.
Hoang, P.T. et al. (2018) Subtype Diversification and Synaptic Specificity of Stem Cell-Derived Spinal Interneurons.
Neuron. 100 (1): 135-149.e7.
Matsusaka, K. et al. (2022) Distinct roles in phagocytosis of the early and late increases of cell surface calreticulin induced by oxaliplatin
Biochem Biophys Rep. 29: 101222.
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0001530 lipopolysaccharide binding
- GO:0001847 opsonin receptor activity
- GO:0006915 apoptosis
- GO:0006909 phagocytosis
- GO:0006954 inflammatory response
- GO:0008063 Toll signaling pathway
- GO:0031225 anchored to membrane
- GO:0016019 peptidoglycan receptor activity
- View More GO Terms
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0045087 innate immune response
- GO:0070891 lipoteichoic acid binding
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071223 cellular response to lipoteichoic acid
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