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CD103 antibody | LF61

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Mouse anti Human CD103:StarBright Violet 570

Product Type
Monoclonal Antibody
Clone
LF61
Isotype
IgG1
Specificity
CD103

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New MCA1416SBV570
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F 100 Tests/0.5ml loader
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Mouse anti Human CD103 antibody, clone LF61 recognizes the human CD103 cell surface antigen, a glycoprotein expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alpha E integrin.

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Violet 570 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Hairy cell leukemia cells.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 570 404 571
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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Source Reference

  1. Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150 kDa, 125 kDa, 105 kDa) associated with hairy cell leukaemia.
    Br J Haematol. 76 (4): 451-9.

References for CD103 antibody

  1. Falini, B. et al. (1990) Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients.
    Br J Haematol. 76 (4): 460-8.
  2. Ling, K.L. et al. (2007) Modulation of CD103 expression on human colon carcinoma-specific CTL.
    J Immunol. 178: 2908-15.
  3. Hadley, G.A. et al. (2001) CD103+ CTL accumulate within the graft epithelium during clinical renal allograft rejection.
    Transplantation. 72 (9): 1548-55.

UniProt
P38570
Entrez Gene
ITGAE
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway

MCA1416SBV570

100008256 100008257

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