C3a Receptor antibody | hC3aRZ1
Mouse anti Human C3a Receptor:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- hC3aRZ1
- Isotype
- IgG1
- Specificity
- C3a Receptor
Mouse anti Human C3a Receptor antibody, clone hC3aRZ1 recognizes the receptor for human complement component C3a (C3aR), a ~55 kDa cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes. Expression levels of C3aR on human cells varies significantly between donors. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Recombinant human C3aR protein.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
Source Reference
-
Zwirner, J. et al. (1999) Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies.
Immunology. 97: 166-72.
References for C3a Receptor antibody
-
Werfel, T. et al. (2000) Activated human T lymphocytes express a functional C3a receptor.
J Immunol. 165 (11): 6599-605. -
Kacani, L. et al. (2001) C5a and C5a(desArg) enhance the susceptibility of monocyte-derived macrophages to HIV infection.
J Immunol. 166: 3410-5. -
Soruri, A. et al. (2003) IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo.
J Immunol. 170 (6): 3306-14. -
Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
J Immunol. 183 (1): 506-17. -
Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
J Am Soc Nephrol. 20: 289-98. -
Martel, C. et al. (2011) Requirements for membrane attack complex formation and anaphylatoxins binding to collagen-activated platelets.
PLoS One. 6: e18812. -
Török K (2012) Human T cell derived, cell-bound complement iC3b is integrally involved in T cell activation.
Immunol Lett. 143: 131-6. -
Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
Atherosclerosis. 238 (2): 289-95.
View The Latest Product References
-
Hengartner, N.E. et al. (2015) Crucial Role of IL1beta and C3a in the In Vitro-Response of Multipotent Mesenchymal Stromal Cells to Inflammatory Mediators of Polytrauma.
PLoS One. 10: e0116772.
- Synonyms
- Complement Component 3a Receptor 1
- RRID
- AB_1100497
- UniProt
- Q16581
- Entrez Gene
- C3AR1
- GO Terms
- GO:0004435 phosphatidylinositol phospholipase C activity
- GO:0004876 complement component C3a receptor activity
- GO:0004943 C3a anaphylatoxin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0008015 blood circulation
MCA2455A647T
153265If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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