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C3a Receptor antibody | hC3aRZ1

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Anti Human C3a Receptor Antibody, clone hC3aRZ1 thumbnail image 1
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Mouse anti Human C3a receptor antibody, clone hC3aRZ1 used for flow cytometry.
Image caption
Expression of C3aR1 and C5aR on collagen- and convulxin-activated platelets. Washed platelets were activated for 10 min with 2.5 µg/mL collagen, 500 ng/mL convulxin or saline and further incubated for 30 min at RT and analyzed for C3aR1 and C5aR expression by flow cytometry. A: representative overlays of fluorescence histograms obtained for each condition using an alexa fluor 488 anti-C3aR1 (solid line), a FITC anti-CD88 (dashed line) or a FITC control IgG (dotted line). B: Mean fluorescence intensities ± SEM of 3 to 9 experiments for each condition.

From: Martel C, Cointe S, Maurice P, Matar S, Ghitescu M, et al. (2011)
Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets.
PLoS ONE 6(4): e18812.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human C3a Receptor:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
hC3aRZ1
Isotype
IgG1
Specificity
C3a Receptor

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Product Code Applications Pack Size List Price Your Price Qty
MCA2455A488
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F 100 Tests/1ml loader
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Mouse anti Human C3a Receptor antibody, clone hC3aRZ1 recognizes the receptor for human complement component C3a (C3aR), a ~55 kDa cell surface protein of the rhodopsin family of G protein-coupled seven-transmembrane receptors. C3a is an anaphylatoxin, which is a potent proinflammatory mediator that possesses immunoregulatory properties. C3a regulates inflammatory functions by binding to the C3a receptor. C3aR is expressed on various tissues and leucocytes including basophils, neutrophils, mast cells, eosinophils and activated T lymphocytes.

Expression levels of C3aR on human cells varies significantly between donors.

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor®488 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Recombinant human C3aR protein.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the sugested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 F 100 Tests/1ml loader
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Description Mouse IgG1 Negative Control:Alexa Fluor® 488

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

Source Reference

  1. Zwirner, J. et al. (1999) Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies.
    Immunology. 97: 166-72.

References for C3a Receptor antibody

  1. Werfel, T. et al. (2000) Activated human T lymphocytes express a functional C3a receptor.
    J Immunol. 165 (11): 6599-605.
  2. Kacani, L. et al. (2001) C5a and C5a(desArg) enhance the susceptibility of monocyte-derived macrophages to HIV infection.
    J Immunol. 166: 3410-5.
  3. Soruri, A. et al. (2003) IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo.
    J Immunol. 170 (6): 3306-14.
  4. Raby, A.C. et al. (2009) Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering.
    J Immunol. 183 (1): 506-17.
  5. Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
    J Am Soc Nephrol. 20: 289-98.
  6. Martel, C. et al. (2011) Requirements for membrane attack complex formation and anaphylatoxins binding to collagen-activated platelets.
    PLoS One. 6: e18812.
  7. Török K (2012) Human T cell derived, cell-bound complement iC3b is integrally involved in T cell activation.
    Immunol Lett. 143: 131-6.
  8. Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
    Atherosclerosis. 238 (2): 289-95.
    9. View The Latest Product References
  9. Hengartner, N.E. et al. (2015) Crucial Role of IL1beta and C3a in the In Vitro-Response of Multipotent Mesenchymal Stromal Cells to Inflammatory Mediators of Polytrauma.
    PLoS One. 10: e0116772.

Flow Cytometry

Synonyms
Complement Component 3a Receptor 1
RRID
AB_877451
UniProt
Q16581
Entrez Gene
C3AR1
GO Terms
GO:0004435 phosphatidylinositol phospholipase C activity
GO:0004876 complement component C3a receptor activity
GO:0004943 C3a anaphylatoxin receptor activity
GO:0005887 integral to plasma membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0008015 blood circulation

MCA2455A488

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