B Cells antibody | FMC7

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Flow Cytometry

Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.

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Human Seroblock	BUF070A	F	50 Test		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Human Seroblock
Human Seroblock	BUF070B	F	200 Test		Log in	- +	Add to Cart Add to Quote Get Quote
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Source Reference

1. Brooks, D.A. et al. (1981) Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. IV. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes.
   J Immunol. 126 (4): 1373-7.

References for B Cells antibody

1. Catovsky, D. et al. (1981) Heterogeneity of B-cell leukemias demonstrated by the monoclonal antibody FMC7.
   Blood. 58 (2): 406-8.
2. Serke, S. et al. (2001) Monoclonal antibody FMC7 detects a conformational epitope on the CD20 molecule: Evidence from phenotyping after Rituxan therapy and transfectant cell analyses.
   Cytometry (Comm. Clin. Cytometry) 46:98-104
3. Zola H., et al. (1984) The human B cell lineage studied with monoclonal antibodies.
   In Leucocyte Typing Ed.A. Bernard, Springer Verlag. p363-71.
4. Zola, H. et al. (1984) The antigen of mature human B cells detected by the monoclonal antibody FMC7: studies on the nature of the antigen and modulation of its expression.
   J Immunol. 133 (1): 321-6.
5. Bloem, A.C. et al. (1988) Functional properties of human B cell subpopulations defined by monoclonal antibodies HB4 and FMC7.
   J Immunol. 140 (3): 768-73.
6. Zola, H. et al. (1987) Markers of differentiated B cell leukaemia: CD22 antibodies and FMC7 react with different molecules.
   Dis Markers. 5 (4): 227-35.
7. Ghia, P. et al. (2003) The pattern of CD38 expression defines a distinct subset of chronic lymphocytic leukemia (CLL) patients at risk of disease progression.
   Blood. 101 (4): 1262-9.
8. Ferro LM & Zola H (1990) Modulation of expression of the antigen identified by FMC7 upon human B-lymphocyte activation: evidence for differences between activation in vivo and in vitro.
   Immunology. 69 (3): 373-8.
View The Latest Product References
9. Collins, R.J. et al. (1983) Malignant lymphoma: reactive with the monoclonal antibody fmc-7
   Pathology. 15 (3): 350-1. (Conference abstract).
10. Zucchetto A et al. (2006) A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia.
    J Cell Physiol. 207 (2): 354-63.
11. Wang, C. et al. (2002) Differentiation of monoclonal B lymphocytosis of undetermined significance (MLUS) and chronic lymphocytic leukemia (CLL) with weak CD5 expression from CD5(-) CLL.
    Leuk Res. 26 (12): 1125-9.
12. Amato, D. et al. (2007) Cytogenetic aberrations and immunoglobulin VH gene mutations in clinically benign CD5- monoclonal B-cell lymphocytosis.
    Am J Clin Pathol. 128 (2): 333-8.
13. Polyak, M.J. et al. (2003) A cholesterol-dependent CD20 epitope detected by the FMC7 antibody.
    Leukemia. 17 (7): 1384-9.
14. Domingo-Domènech, E. et al. (2002) CD38 expression in B-chronic lymphocytic leukemia: association with clinical presentation and outcome in 155 patients.
    Haematologica. 87 (10): 1021-7.
15. Gladkikh, A. et al. (2010) Cyclin D1 expression in B-cell lymphomas.
    Exp Hematol. 38 (11): 1047-57.
16. Unruh, T.L. et al. (2005) Cholesterol depletion inhibits src family kinase-dependent calcium mobilization and apoptosis induced by rituximab crosslinking.
    Immunology. 116 (2): 223-32.
17. Gladkikh, A.A. et al. (2017) Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70.
    Cancer Med. 6 (12): 2984-97.

Synonyms

CD20

RRID

AB_321192

UniProt

P11836

Entrez Gene

MS4A1

GO Terms

GO:0005887 integral to plasma membrane

GO:0006955 immune response

GO:0042113 B cell activation

MCA792F

150887 163402 164705 164997

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