Actin Beta antibody | AbD12141
Human anti Human Actin Beta:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD12141
- Isotype
- HuCAL Fab bivalent
- Specificity
- Actin Beta
Human anti human actin beta antibody, clone AbD12141 recognizes the 42 kDa product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modelling. Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used Western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Vertebrates Expected from Sequence Invertebrates Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to Horseradish Peroxidase (HRP) - liquid.
- Preparation
- StrepTactin affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified human beta-actin
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- Western Blotting
- Human anti Human actin beta antibody, clone AbD12141 is suitable for use as a loading control.
References for Actin Beta antibody
-
Walsh, C.A. et al. (2020) The mevalonate precursor enzyme HMGCS1 is a novel marker and key mediator of cancer stem cell enrichment in luminal and basal models of breast cancer.
PLoS One. 15 (7): e0236187. -
Lederhofer, J. et al. (2018) Development of a Virosomal RSV Vaccine Containing 3D-PHAD® Adjuvant: Formulation, Composition, and Long-Term Stability.
Pharm Res. 35 (9): 172.
- Synonyms
- ACTB
- RRID
- AB_10964917
- UniProt
- P60709
- Entrez Gene
- ACTB
HCA147P
151293 155286 159100 168204If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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