CD23 antibody | 51-3
Mouse anti Horse CD23:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 51-3
- Isotype
- IgG1
- Specificity
- CD23
Antibody Quality and Performance Guarantee
MCA6394A647
| Mouse anti Horse CD23 antibody, clone 51-3 recognizes horse CD23 also known as Fc epsilon Receptor II (FcΕRII). CD23, a C-type lectin, is the low affinity receptor for IgE, but also binds CD21, MHC class II and the integrin alpha-chains CD11b and CD11c. The extracellular domain can be released by proteolytic cleavage and bind ligands such as CD21. In humans and mice, CD23 is found on B-cell subsets and monocytes with low levels of expression on T-cells, follicular dendritic cells, eosinophils, NK-cells, Langerhans cells and platelets (Conrad, 1990). Key functions for CD23 include negative regulation for IgE and IgG antibody responses in B cells by influencing cellular activation and differentiation (Liu et al. 2016) and modulating allergic disease through IgE-facilitated allergen presentation, controlling allergen-specific T-cell activation (Selb et al. 2017). Mouse anti Horse CD23 antibody, clone 51-3, identified small lymphocytes expressing high levels of MHC class II which were also positive for surface IgE. CD23 was barely detectable on blood T-cells or monocytes. A population with a median CD23 expression of 2.5% (range 0.9–4.5%) was found in equine PBMC (Wagner et al. 2012). |
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to Alexa Fluor®647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Recombinant equine CD23/IL-4 fusion protein
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X3.Ag8-653 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Antibody Characterization Reference
-
Wagner, B. et al. (2012) Monoclonal antibodies to equine CD23 identify the low-affinity receptor for IgE on subpopulations of IgM+ and IgG1+ B-cells in horses.
Vet Immunol Immunopathol. 146 (2): 125-34.
- UniProt
- A0A3Q2H947
MCA6394A647
165387
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