CD4 antibody | CT7
Mouse anti Guinea Pig CD4 antibody, clone CT7 (MCA749S) used for the detection of CD4 expressing cells in the guinea pig airway by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Immunohistochemistry staining for CD4+ cells was not increased in animals neonatally sensitized compared with control animals. Representative slides of airway sections from adult animals immunostained for CD4+ cells are shown in the top and middle panels. The arrow shows an area of the epithelium with intense positive staining for CD4. Computerized quantification of CD4+ staining, expressed as % area of the airway wall, in control guinea pigs and animals sensitized at birth is shown in the bottom panel as means and standard errors, n = 5, n = 5, and n = 5 for 1‐week, 3‐week, and adult control animals, n = 5, n = 6, and n = 5 for 1‐week, 3‐week, and adult sensitized animals, respectively. A significant reduction was observed in the tissue of the 3‐week‐sensitized group compared to 3‐week controls (P < 0.05).
From: Chitano P, Wang L, Degan S, Worthington CL, Pozzato V, Hussaini SH, Turner WC, Dorscheid DR, Murphy TM.
Ovalbumin sensitization of guinea pig at birth prevents the ontogenetic decrease in airway smooth muscle responsiveness.
Physiol Rep. 2014 Dec 11;2(12). pii: e12241.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Guinea Pig CD4 antibody, clone CT7 (MCA749S) used for the detection of CD4 expressing cells in the guinea pig lungs by flow cytometry
Image caption:
Monitoring of activated T cells in the infected lungs during therapy. Percentage of activated CD4+ T cells (A) and CD8+ T cells (B) in drug or drug plus MIP group were evaluated at different time points during the course of therapy. The percentage of respective cells in infected control group has been shown in the inset of bar diagram. Activated T cells were analyzed based on the expression of homing receptors as shown in the representative contour plots and compared at 15 days and 30 days of therapy. In each plot, region R6 represents T cells with high homing receptor expression and the corresponding percentage value is mentioned inside the region. Data represents the mean value of four animals in each group. * p<0.05. Uninfected animal (naive) was taken as uninfected negative control and untreated animal at 30 days post infection was taken as infected positive control.
From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, et al. (2012)
Efficacy of Mycobacterium indicus pranii Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung.
PLoS ONE 7(7): e39215.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Guinea Pig CD4 antibody, clone CT7 (MCA749S) used for the detection of CD4 expressing cells in the guinea pig lungs by immunohistochemistry on formailin fixed cryosections.
Image caption:
Evaluation of cellular immune response inside the granuloma of infected lungs. Scattergrams show the numbers of CD4+ T cells, CD8+ T cells, granulocytes and MHC II+ cells per unit area of granuloma from different experimental groups after 30 days of therapy. The number of different immune cells inside the granuloma in the lung sections from different experimental groups were counted microscopically and expressed as number of cells per unit area of granuloma. Minimum of 1000 cells were counted from randomly selected, at least 10 fields. Sections were taken from lung samples of 4 animals per group. The representative photomicrographs show the positively stained cells with red arrow in control group, drug or drug + MIP group in each panel. DAB was used as substrate in staining granulocytes while VIP red was substrate for staining other types of cells. * p<0.05, ** p<0.01.
From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, et al. (2012)
Efficacy of Mycobacterium indicus pranii Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung.
PLoS ONE 7(7): e39215.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Guinea Pig CD4 antibody, clone CT7 (MCA749PE) used for the evaluation of CD4 expression on guinea pig lymphocytes by flow cytometry.
Image caption:
BCG-vaccination elicits CD1b-restricted, PIM6-specific T cell-responses. Four weeks after BCG-vaccination PBMCs were isolated. Autologous, CD1b-expressing APCs and non-adherent lymphocytes were stained with CFSE and stimulated with mycobacterial antigens. CFSE dilution was analyzed by flow-cytometry after five days. (B) CFSE-low, PIM6-reactive lymphocytes were stained for a general T cell-marker, CD4 and CD8. The small panel at the left shows the gating strategy. The larger pseudo-color graph representatively shows the CD4- and CD8-distribution. The dot blot on the right shows the relative distribution for T cell-markers in PIM6-reactive cells four weeks after BCG-vaccination. Red symbols represent the normal T cell- and CD4-CD8-distribution in PBMCs from naïve control guinea pigs. Asterisks indicate the level of significance as determined by unpaired t-test.
From: Eckhardt E, Schinköthe J, Gischke M, Sehl-Ewert J, Corleis B, Dorhoi A, Teifke J, Albrecht D, Geluk A, Gilleron M, Bastian M.
Phosphatidylinositolmannoside vaccination induces lipid-specific Th1-responses and partially protects guinea pigs from Mycobacterium tuberculosis challenge.
Sci Rep. 2023 Oct 30;13(1):18613.
doi: 10.1038/s41598-023-45898-3.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Guinea Pig CD4:FITC
- Product Type
- Monoclonal Antibody
- Clone
- CT7
- Isotype
- IgG1
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Mouse anti Guinea Pig CD4 antibody, clone CT7 recognizes the CD4 antigen present on T Helper/Inducer lymphocytes. |
- Target Species
- Guinea Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Guinea pig peritoneal T-cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole Guinea Pig peripheral blood.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for CD4 antibody
-
Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
Hybridoma. 4 (2): 115-24. -
Liversidge, J. & Forrester, J.V. (1988) Experimental autoimmune uveitis (EAU): immunophenotypic analysis of inflammatory cells in chorio retinal lesions.
Curr Eye Res. 7 (12): 1231-41. -
Steerenberg, P.A. et al. (1991) Tumour rejection after adoptive transfer of line-10-immune spleen cells is mediated by two T cell subpopulations.
Cancer Immunol Immunother. 34 (2): 103-10. -
Debout, C. et al. (1991) The Kurloff cell in estrogenized guinea pigs as a CT7+ 8BE6- CT6- MR-1- CT10- IgM- lymphocyte with natural killer activity.
Nat Immun Cell Growth Regul. 10 (6): 327-35. -
Dascher, C.C. et al. (1999) Conservation of a CD1 multigene family in the guinea pig.
J Immunol. 163: 5478-88. -
Hiromatsu, K. et al. (2002) Induction of CD1-restricted immune responses in guinea pigs by immunization with mycobacterial lipid antigens.
J Immunol. 169: 330-9. -
Rousseau, C. et al. (2003) Sulfolipid Deficiency Does Not Affect the Virulence of Mycobacterium tuberculosis H37Rv in Mice and Guinea Pigs
Infect Immun. 71: 4684-90. -
Jeevan, A. et al. (2003) Differential expression of gamma interferon mRNA induced by attenuated and virulent Mycobacterium tuberculosis in guinea pig cells after Mycobacterium bovis BCG vaccination.
Infect Immun. 71: 354-64.
View The Latest Product References
-
Turner, O.C. et al. (2003) Immunopathogenesis of pulmonary granulomas in the guinea pig after infection with Mycobacterium tuberculosis.
Infect Immun. 71: 864-71. -
Schleiss, M.R. et al. (2007) Preconceptual administration of an alphavirus replicon UL83 (pp65 homolog) vaccine induces humoral and cellular immunity and improves pregnancy outcome in the guinea pig model of congenital cytomegalovirus infection.
J Infect Dis.195: 789-98. -
Mishra, N.C. et al. (2010) Sulfur mustard induces immune sensitization in hairless guinea pigs.
Int Immunopharmacol. 10: 193-9. -
Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
Infect Immun. 78: 1670-81. -
Yang H et al. (2011) Three protein cocktails mediate delayed-type hypersensitivity responses indistinguishable from that elicited by purified protein derivative in the guinea pig model of Mycobacterium tuberculosis infection.
Infect Immun. 79 (2): 716-23. -
Komori, T. et al. (2011) A Microbial Glycolipid Functions as a New Class of Target Antigen for Delayed-type Hypersensitivity.
J Biol Chem. 286: 16800-6. -
Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
Infect Immun. 79 (5): 1889-97. -
Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
Antimicrob Agents Chemother. 55 (1): 124-31. -
Kramp, J.C. et al. (2011) The in vivo immunomodulatory effect of recombinant tumour necrosis factor-alpha in guinea pigs vaccinated with Mycobacterium bovis bacille Calmette-Guérin.
Clin Exp Immunol. 165: 110-20. -
Gupta, A. et al. (2012) Efficacy of Mycobacterium indicus pranii immunotherapy as an adjunct to chemotherapy for tuberculosis and underlying immune responses in the lung.
PLoS One. 7 (7): e39215. -
Shang, S. et al. (2012) Drug treatment combined with BCG vaccination reduces disease reactivation in guinea pigs infected with Mycobacterium tuberculosis.
Vaccine. 30 (9): 1572-82. -
Jeevan A et al. (2013) Guinea pig skin, a model for epidermal cellular and molecular changes induced by UVR in vivo and in vitro: effects on Mycobacterium bovis Bacillus Calmette-Guérin vaccination.
Photochem Photobiol. 89 (1): 189-98. -
Chitano, P. et al. (2014) Ovalbumin sensitization of guinea pig at birth prevents the ontogenetic decrease in airway smooth muscle responsiveness.
Physiol Rep. 2 (12): e12241. -
Podell, B.K .et al. (2014) Increased severity of tuberculosis in Guinea pigs with type 2 diabetes: a model of diabetes-tuberculosis comorbidity.
Am J Pathol. 184 (4): 1104-18. -
Miszczyk, E. et al. (2014) Antigen-specific lymphocyte proliferation as a marker of immune response in guinea pigs with sustained Helicobacter pylori infection.
Acta Biochim Pol. 61 (2): 295-303. -
Eckhardt, E. et al. (2023) Phosphatidylinositolmannoside vaccination induces lipid-specific Th1-responses and partially protects guinea pigs from Mycobacterium tuberculosis challenge.
Sci Rep. 13 (1): 18613.
- RRID
- AB_322605
MCA749F
159779
1602
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