CD4 antibody | CT7

Mouse anti Guinea Pig CD4:FITC

Product Type
Monoclonal Antibody
Clone
CT7
Isotype
IgG1
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA749F
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Mouse anti Guinea Pig CD4 antibody, clone CT7 recognizes the CD4 antigen present on T Helper/Inducer lymphocytes.

Target Species
Guinea Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Guinea pig peritoneal T-cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole Guinea Pig peripheral blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

References for CD4 antibody

  1. Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
    Hybridoma. 4 (2): 115-24.
  2. Baker, D. et al. (1987) Changes in lymphocyte subsets after treatment with cyclophosphamide and during the development of contact sensitivity in the guinea pig.
    Int J Immunopharmacol. 9 (2): 175-83.
  3. Liversidge, J. & Forrester, J.V. (1988) Experimental autoimmune uveitis (EAU): immunophenotypic analysis of inflammatory cells in chorio retinal lesions.
    Curr Eye Res. 7 (12): 1231-41.
  4. Steerenberg, P.A. et al. (1991) Tumour rejection after adoptive transfer of line-10-immune spleen cells is mediated by two T cell subpopulations.
    Cancer Immunol Immunother. 34 (2): 103-10.
  5. Debout, C. et al. (1991) The Kurloff cell in estrogenized guinea pigs as a CT7+ 8BE6- CT6- MR-1- CT10- IgM- lymphocyte with natural killer activity.
    Nat Immun Cell Growth Regul. 10 (6): 327-35.
  6. Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
    Antimicrob Agents Chemother. 55 (1): 124-31.
  7. Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
    Infect Immun. 79 (5): 1889-97.
  8. Komori, T. et al. (2011) A Microbial Glycolipid Functions as a New Class of Target Antigen for Delayed-type Hypersensitivity.
    J Biol Chem. 286: 16800-6.
  9. View The Latest Product References
  10. Jeevan, A. et al. (2003) Differential expression of gamma interferon mRNA induced by attenuated and virulent Mycobacterium tuberculosis in guinea pig cells after Mycobacterium bovis BCG vaccination.
    Infect Immun. 71: 354-64.
  11. Schleiss, M.R. et al. (2007) Preconceptual administration of an alphavirus replicon UL83 (pp65 homolog) vaccine induces humoral and cellular immunity and improves pregnancy outcome in the guinea pig model of congenital cytomegalovirus infection.
    J Infect Dis.195: 789-98.
  12. Turner, O.C. et al. (2003) Immunopathogenesis of pulmonary granulomas in the guinea pig after infection with Mycobacterium tuberculosis.
    Infect Immun. 71: 864-71.
  13. Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
    Infect Immun. 78: 1670-81.
  14. Mishra, N.C. et al. (2010) Sulfur mustard induces immune sensitization in hairless guinea pigs.
    Int Immunopharmacol. 10: 193-9.
  15. Hiromatsu, K. et al. (2002) Induction of CD1-restricted immune responses in guinea pigs by immunization with mycobacterial lipid antigens.
    J Immunol. 169: 330-9.
  16. Dascher, C.C. et al. (1999) Conservation of a CD1 multigene family in the guinea pig.
    J Immunol. 163: 5478-88.
  17. Rousseau, C. et al. (2003) Sulfolipid Deficiency Does Not Affect the Virulence of Mycobacterium tuberculosis H37Rv in Mice and Guinea Pigs
    Infect Immun. 71: 4684-90.
  18. Kramp, J.C. et al. (2011) The in vivo immunomodulatory effect of recombinant tumour necrosis factor-alpha in guinea pigs vaccinated with Mycobacterium bovis bacille Calmette-Guérin.
    Clin Exp Immunol. 165: 110-20.
  19. Chitano, P. et al. (2014) Ovalbumin sensitization of guinea pig at birth prevents the ontogenetic decrease in airway smooth muscle responsiveness.
    Physiol Rep. 2 (12)Dec 11 [Epub ahead of print].
  20. Gupta, A. et al. (2012) Efficacy of Mycobacterium indicus pranii immunotherapy as an adjunct to chemotherapy for tuberculosis and underlying immune responses in the lung.
    PLoS One. 7 (7): e39215.
  21. Podell, B.K .et al. (2014) Increased severity of tuberculosis in Guinea pigs with type 2 diabetes: a model of diabetes-tuberculosis comorbidity.
    Am J Pathol. 184 (4): 1104-18.
  22. Shang, S. et al. (2012) Drug treatment combined with BCG vaccination reduces disease reactivation in guinea pigs infected with Mycobacterium tuberculosis.
    Vaccine. 30 (9): 1572-82.
  23. Yang H et al. (2011) Three protein cocktails mediate delayed-type hypersensitivity responses indistinguishable from that elicited by purified protein derivative in the guinea pig model of Mycobacterium tuberculosis infection.
    Infect Immun. 79 (2): 716-23.
  24. Jeevan A et al. (2013) Guinea pig skin, a model for epidermal cellular and molecular changes induced by UVR in vivo and in vitro: effects on Mycobacterium bovis Bacillus Calmette-Guérin vaccination.
    Photochem Photobiol. 89 (1): 189-98.
  25. Miszczyk, E. et al. (2014) Antigen-specific lymphocyte proliferation as a marker of immune response in guinea pigs with sustained Helicobacter pylori infection.
    Acta Biochim Pol. 61 (2): 295-303.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

RRID
AB_322605
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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