Rhizopus arrhizus antibody | WSSA-RA-1
Mouse anti Rhizopus arrhizus antibody, clone WSSA-RA-1 recognizes Rhizopus arrhizus and other members of the family Mucoraceae including Absidia corymbifera and Rhizomucor pusillus, reacting strongly with the cytoplasm of hyphae and also possibly with the walls and septae, where present.
R. arrhizus, an angio-invasive filamentous fungus, is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease. Clone WSSA-RA-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components.
Clone WSSA-RA-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.
R. arrhizus, an angio-invasive filamentous fungus, is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease. Clone WSSA-RA-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components.
Clone WSSA-RA-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.
Product Details
- Target Species
- Fungal
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Water-soluble somatic antigens (WSSA) from Rhizopus arrhizus.
- Approx. Protein Concentrations
- IgM concentration 1.0mg/ml.
- Fusion Partners
- Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Rhizopus arrhizus antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin 1 | 1/50 | ||
| Immunoprecipitation | |||
| Western Blotting |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Lymph nodes from R.arrhizus infected cattle.
- Western Blotting
- Mouse anti Rhizopus arrhizus detects a number diffuse band/s of between ~14-110kDa of Rhizopus arrhizus water-soluble somatic antigens (Jensen et al. 1996).
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
|
|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
|
|
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
|
|
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
|
Product Specific References
References for Rhizopus arrhizus antibody
-
Jensen, H.E. et al. (1996) Immunohistochemical diagnosis of systemic bovine zygomycosis by murine monoclonal antibodies.
Vet Pathol. 33 (2): 176-83. -
Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests.
APMIS. 104 (4): 241-58. -
Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies.
J Pathol. 181 (1): 100-5. -
Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis.
J Vet Diagn Invest. 8 (1): 68-75. -
Arendrup, M.C. et al. (2009) Breakthrough Aspergillus fumigatus and Candida albicans double infection during caspofungin treatment: laboratory characteristics and implication for susceptibility testing.
Antimicrob Agents Chemother. 53: 1185-93. -
Yasuda, M. et al (2012) A case of intestinal mucormycosis in a common marmoset (Callithrix jacchus).
J Vet Med Sci. 74: 357-9. -
Galiza G.J.N. et al. (2014) Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals.
Pesquisa Veterinária Brasileira. 34 (7): 637-642. -
Suzuta F et al. (2015) Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus).
J Vet Med Sci. 77 (8): 1029-31. -
Nishimura, M. et al. (2014) Zygomycotic mediastinal lymphadenitis in beef cattle with ruminal tympany.
J Vet Med Sci. 76 (1): 123-7. -
Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
Avian Dis. 60 (3): 705-8.
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