Rhizopus arrhizus antibody | WSSA-RA-1
R. arrhizus, an angio-invasive filamentous fungus, is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease. Clone WSSA-RA-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components.
Clone WSSA-RA-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.
- Target Species
- Product Form
- Purified IgM - liquid
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
0.09% Sodium Azide (NaN3)
- Water-soluble somatic antigens (WSSA) from Rhizopus arrhizus.
- Approx. Protein Concentrations
- IgM concentration 1.0mg/ml.
- Fusion Partners
- Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
Applications of Rhizopus arrhizus antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/50|
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.
- Histology Positive Control Tissue
- Lymph nodes from R.arrhizus infected cattle.
- Western Blotting
- Mouse anti Rhizopus arrhizus detects a number diffuse band/s of between ~14-110kDa of Rhizopus arrhizus water-soluble somatic antigens (Jensen et al. 1996).
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Human anti Mouse IgM:FITC||HCA040F||F||0.1 mg|
|Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)||STAR138A||C E P WB||1 ml|
|Goat anti Mouse IgG/A/M:Alk. Phos.||STAR87A||C E WB||1 mg|
|Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)||STAR87P||E||1 mg|
Product Specific References
References for Rhizopus arrhizus antibody
Jensen, H.E. et al. (1996) Immunohistochemical diagnosis of systemic bovine zygomycosis by murine monoclonal antibodies.
Vet Pathol. 33 (2): 176-83.
Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests.
APMIS. 104 (4): 241-58.
Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies.
J Pathol. 181 (1): 100-5.
Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis.
J Vet Diagn Invest. 8 (1): 68-75.
Arendrup, M.C. et al. (2009) Breakthrough Aspergillus fumigatus and Candida albicans double infection during caspofungin treatment: laboratory characteristics and implication for susceptibility testing.
Antimicrob Agents Chemother. 53: 1185-93.
Yasuda, M. et al (2012) A case of intestinal mucormycosis in a common marmoset (Callithrix jacchus).
J Vet Med Sci. 74: 357-9.
Galiza G.J.N. et al. (2014) Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals.
Pesquisa Veterinária Brasileira. 34 (7): 637-642.
Suzuta F et al. (2015) Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus).
J Vet Med Sci. 77 (8): 1029-31.
Nishimura, M. et al. (2014) Zygomycotic mediastinal lymphadenitis in beef cattle with ruminal tympany.
J Vet Med Sci. 76 (1): 123-7.
Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
Avian Dis. 60 (3): 705-8.