Rhizopus arrhizus antibody | WSSA-RA-1

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Mouse anti Rhizopus arrhizus

Product Type
Monoclonal Antibody
Clone
WSSA-RA-1
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2577 E P* WB 0.25 mg
Mouse anti Rhizopus arrhizus antibody, clone WSSA-RA-1 recognizes Rhizopus arrhizus and other members of the family Mucoraceae including Absidia corymbifera and Rhizomucor pusillus, reacting strongly with the cytoplasm of hyphae and also possibly with the walls and septae, where present.

R. arrhizus, an angio-invasive filamentous fungus, is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease. Clone WSSA-RA-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components.

Clone WSSA-RA-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.

Product Details

Target Species
Fungal
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Water-soluble somatic antigens (WSSA) from Rhizopus arrhizus.
Approx. Protein Concentrations
IgM concentration 1.0mg/ml.
Fusion Partners
Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Rhizopus arrhizus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/50
Immunoprecipitation
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Lymph nodes from R.arrhizus infected cattle.
Western Blotting
Mouse anti Rhizopus arrhizus detects a number diffuse band/s of between ~14-110kDa of Rhizopus arrhizus water-soluble somatic antigens (Jensen et al. 1996).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Rhizopus arrhizus antibody

  1. Jensen, H.E. et al. (1996) Immunohistochemical diagnosis of systemic bovine zygomycosis by murine monoclonal antibodies.
    Vet Pathol. 33 (2): 176-83.
  2. Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests.
    APMIS. 104 (4): 241-58.
  3. Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies.
    J Pathol. 181 (1): 100-5.
  4. Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis.
    J Vet Diagn Invest. 8 (1): 68-75.
  5. Arendrup, M.C. et al. (2009) Breakthrough Aspergillus fumigatus and Candida albicans double infection during caspofungin treatment: laboratory characteristics and implication for susceptibility testing.
    Antimicrob Agents Chemother. 53: 1185-93.
  6. Yasuda, M. et al (2012) A case of intestinal mucormycosis in a common marmoset (Callithrix jacchus).
    J Vet Med Sci. 74: 357-9.
  7. Galiza G.J.N. et al. (2014) Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals.
    Pesquisa VeterinĂ¡ria Brasileira. 34 (7): 637-642.
  8. Suzuta F et al. (2015) Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus).
    J Vet Med Sci. 77 (8): 1029-31.
  9. Nishimura, M. et al. (2014) Zygomycotic mediastinal lymphadenitis in beef cattle with ruminal tympany.
    J Vet Med Sci. 76 (1): 123-7.
  10. Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
    Avian Dis. 60 (3): 705-8.