Rhizopus arrhizus antibody | WSSA-RA-1
R. arrhizus, an angio-invasive filamentous fungus, is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease. Clone WSSA-RA-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components.
Clone WSSA-RA-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.
Product Details
- Target Species
- Fungal
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Water-soluble somatic antigens (WSSA) from Rhizopus arrhizus.
- Approx. Protein Concentrations
- IgM concentration 1.0mg/ml.
- Fusion Partners
- Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Rhizopus arrhizus antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Paraffin 1 | 1/50 | ||
Immunoprecipitation | |||
Western Blotting |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.
- Histology Positive Control Tissue
- Lymph nodes from R.arrhizus infected cattle.
- Western Blotting
- Mouse anti Rhizopus arrhizus detects a number diffuse band/s of between ~14-110kDa of Rhizopus arrhizus water-soluble somatic antigens (Jensen et al. 1996).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
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Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
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Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
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Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
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Product Specific References
References for Rhizopus arrhizus antibody
-
Jensen, H.E. et al. (1996) Immunohistochemical diagnosis of systemic bovine zygomycosis by murine monoclonal antibodies.
Vet Pathol. 33 (2): 176-83. -
Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests.
APMIS. 104 (4): 241-58. -
Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies.
J Pathol. 181 (1): 100-5. -
Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis.
J Vet Diagn Invest. 8 (1): 68-75. -
Arendrup, M.C. et al. (2009) Breakthrough Aspergillus fumigatus and Candida albicans double infection during caspofungin treatment: laboratory characteristics and implication for susceptibility testing.
Antimicrob Agents Chemother. 53: 1185-93. -
Yasuda, M. et al (2012) A case of intestinal mucormycosis in a common marmoset (Callithrix jacchus).
J Vet Med Sci. 74: 357-9. -
Galiza G.J.N. et al. (2014) Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals.
Pesquisa Veterinária Brasileira. 34 (7): 637-642. -
Suzuta F et al. (2015) Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus).
J Vet Med Sci. 77 (8): 1029-31. -
Nishimura, M. et al. (2014) Zygomycotic mediastinal lymphadenitis in beef cattle with ruminal tympany.
J Vet Med Sci. 76 (1): 123-7. -
Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
Avian Dis. 60 (3): 705-8.
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