Aspergillus antibody | WF-AF-1
Mouse anti Aspergillus spp., clone WF-AF-1, is raised against the wall fraction (WF) of Aspergillus fumigatus. This antibody specifically recognises members of the Aspergillus spp. including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of hyphae.
A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. Clone WF-AF-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. Clone WF-AF-1 has also been used for the identification of aspergillosis in human tissue sections.
Clone WF-AF-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium.
A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. Clone WF-AF-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. Clone WF-AF-1 has also been used for the identification of aspergillosis in human tissue sections.
Clone WF-AF-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium.
Product Details
- Target Species
- Fungal
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ammonium sulfate precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Wall fraction (WF) of Aspergillus fumigatus
- Approx. Protein Concentrations
- IgM concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Aspergillus antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin 1 | 1/300 | ||
| Immunoprecipitation | |||
| Western Blotting |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Aspergillus infected placenta.
- Western Blotting
- Mouse anti Aspergillus spp. antibody, clone WF-AF-1 detects a band of approximately 106kDa of Aspergillus fumigatus wall fraction (WF).
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | 0.1 mg | F | ||
| Human anti Mouse IgM:HRP | HCA040P | 0.1 mg | E | ||
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | 1 ml | C E P WB | ||
| Goat anti Mouse IgM:RPE | STAR86PE | 0.5 ml | F | ||
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | 1 mg | C E WB | ||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | 1 mg | E |
Product Specific References
References for Aspergillus antibody
-
Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis.
J Vet Diagn Invest. 8 (1): 68-75. -
Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests.
APMIS. 104 (4): 241-58. -
Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies.
J Pathol. 181 (1): 100-5. -
Delaney, M.A. et al. (2013) Occlusive fungal tracheitis in 4 captive bottlenose dolphins (Tursiops truncatus).
Vet Pathol. 50 (1): 172-6. -
Goodpaster, T. & Randolph-Habecker, J. (2014) A flexible mouse-on-mouse immunohistochemical staining technique adaptable to biotin-free reagents, immunofluorescence, and multiple antibody staining.
J Histochem Cytochem. 62 (3): 197-204. -
Galiza Glauco J.N. et al. (2014) Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals
Pesquisa Veterinária Brasileira. 34 (7): 637-642. -
Murase, H. et al. (2015) A clinical case of equine fungal placentitis with reference to hormone profiles and ultrasonography.
J Equine Sci. 26 (4): 129-33. -
Dagleish, M.P. et al. (2010) Immunohistochemical diagnosis of infectious diseases of sheep.
Small Ruminant Research. 92 (1-3): 19-35. -
Suzuta F et al. (2015) Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus).
J Vet Med Sci. 77 (8): 1029-31. -
Jin, J-H. et al. (2015) Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors
RSC Adv. 5 (20): 15728-15735. -
Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
Avian Dis. 60 (3): 705-8.
