Aspergillus antibody | WF-AF-1

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Mouse anti Aspergillus SPP

Product Type
Monoclonal Antibody
Clone
WF-AF-1
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2576 E IP P* WB 0.25 mg
Mouse anti Aspergillus spp., clone WF-AF-1, is raised against the wall fraction (WF) of Aspergillus fumigatus. This antibody specifically recognises members of the Aspergillus spp. including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of hyphae.

A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. Clone WF-AF-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. Clone WF-AF-1 has also been used for the identification of aspergillosis in human tissue sections.

Clone WF-AF-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium.

Product Details

Target Species
Fungal
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ammonium sulfate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Wall fraction (WF) of Aspergillus fumigatus
Approx. Protein Concentrations
IgM concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Aspergillus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/300
Immunoprecipitation
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Aspergillus infected placenta.
Western Blotting
Mouse anti Aspergillus spp. antibody, clone WF-AF-1 detects a band of approximately 106kDa of Aspergillus fumigatus wall fraction (WF).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Aspergillus antibody

  1. Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis.
    J Vet Diagn Invest. 8 (1): 68-75.
  2. Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests.
    APMIS. 104 (4): 241-58.
  3. Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies.
    J Pathol. 181 (1): 100-5.
  4. Delaney, M.A. et al. (2013) Occlusive fungal tracheitis in 4 captive bottlenose dolphins (Tursiops truncatus).
    Vet Pathol. 50 (1): 172-6.
  5. Goodpaster, T. & Randolph-Habecker, J. (2014) A flexible mouse-on-mouse immunohistochemical staining technique adaptable to biotin-free reagents, immunofluorescence, and multiple antibody staining.
    J Histochem Cytochem. 62 (3): 197-204.
  6. Galiza Glauco J.N. et al. (2014) Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals
    Pesquisa Veterinária Brasileira. 34 (7): 637-642.
  7. Murase, H. et al. (2015) A clinical case of equine fungal placentitis with reference to hormone profiles and ultrasonography.
    J Equine Sci. 26 (4): 129-33.
  8. Dagleish, M.P. et al. (2010) Immunohistochemical diagnosis of infectious diseases of sheep.
    Small Ruminant Research. 92 (1-3): 19-35.
  9. Suzuta F et al. (2015) Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus).
    J Vet Med Sci. 77 (8): 1029-31.
  10. Jin, J-H. et al. (2015) Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors
    RSC Adv. 5 (20): 15728-15735.
  11. Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
    Avian Dis. 60 (3): 705-8.