CD8 Alpha antibody | Du CD8-1
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|Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 recognizes Pekin duck CD8 alpha (CD8a). CD8a is expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells.
Most avian immunity research has been carried out on chickens and relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin.
Double-staining with clone Du CD8-1 and MCA2480 Mouse anti Duck IgY light chain clone 14A3 revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa (Kothlow et al. 1985). Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard (Anas platyrhynchos).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Chicken
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- 293T cells expressing Pekin Duck CD8 alpha.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry||1||10 ug/ml|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)||STAR117F||F||0.5 mg||Log in|
|List Price||Your Price|
|Description||Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)|
References for CD8 Alpha antibody
Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
Dev Comp Immunol. 29 (8): 733-48.
Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.
Vet Res. 43: 56.
Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
Virol J. 8: 214.
Chen, S. et al. (2015) Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose.
Immunobiology. 220 (6): 753-61.
Chen, S. et al. (2015) Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection.
Poult Sci. 94 (1): 17-24.
Chen, S. et al. (2016) Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
Int J Mol Sci. 17(12):1990.
Cornelissen, J.B. et al. (2013) Differences in highly pathogenic avian influenza viral pathogenesis and associated early inflammatory response in chickens and ducks.
Avian Pathol. 42 (4): 347-64.
Wu, Y. et al. (2019) Changes in the small intestine mucosal immune barrier in Muscovy ducklings infected with Muscovy duck reovirus
Veterinary Microbiology. 233: 85-92.
View The Latest Product References
Apinda, N. et al. (2022) Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing Pasteurella multocida OmpH Gene.
Vaccines (Basel). 10 (8)Aug 19 [Epub ahead of print].
Higgins, D.A. & Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.
J Immunol Methods. 106 (1): 135-45.
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