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CD8 Alpha antibody | Du CD8-1

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Anti Duck CD8 Alpha Antibody, clone Du CD8-1 thumbnail image 1 Anti Duck CD8 Alpha Antibody, clone Du CD8-1 thumbnail image 2 Anti Duck CD8 Alpha Antibody, clone Du CD8-1 thumbnail image 3
Anti Duck CD8 Alpha Antibody, clone Du CD8-1 gallery image 1
Published customer image:
Mouse anti Duck CD4 antibody, clone Du CD4-2 (MCA2478) used along with Mouse anti Duck CD8&aplha; antibody, clone Du CD8-1 (MCA2479) for the demonstration of lymphocyte subsets in goose spleen sections by immunohistochemistry using formalin fixed, paraffin embedded tissue sections.
Image caption:
Global characteristics of goslings after infection with GPV and H9N2. (A) Body weight change of GPV- and H9N2-infected goslings from day 0 to 5. ** p≤0.01, *** p≤0.001; (B) the expression level of IL-1β and IL-6 in the spleen tissues of GPV- and H9N2-infected goslings. ** p≤0.01, *** p≤0.001; (C) histological changes in goose spleens infected with GPV and H9N2 at 5 dpi. The spleen tissues from (a) mock-, (b) GPV-, and (c) H9N2-infected goslings were stained with H &E, and diffuse hemorrhage was observed in either of virus-infected spleen sections, scale bar = 100 μm; and (D) Detection of (a) GPV and (b) H9N2 antigen, as well as (c,d) CD4- and (e,f) CD8α-positive cells by immunohistochemical analysis. The dark brown represents positive signs for viral antigen, CD4, or CD8α molecules, scale bar = 100 μm.

From: Chen S, Wang A, Sun L, Liu F, Wang M, Jia R, Zhu D, Liu M, Yang Q, Wu Y, Sun K, Chen X, Cheng A.
Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
Int J Mol Sci. 2016 Dec 1;17(12). pii: E1990.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Duck CD8 Alpha Antibody, clone Du CD8-1 gallery image 2
Published customer image:
Mouse anti Duck CD8 antibody, clone Du CD8-1 (MCA2479) used for the evaluation of lymphocyte populations in PBLs following vaccination by flow cytometry.
Image caption:
T lymphocytes in PBL following DEV UL24 DNA vaccination. 1, 2, 3, 4, 6, 8 weeks after vaccination, the isolated PBL were stained with monoclonal antibodies against duck CD4 (A) and CD8 (B). The results presented are the mean of all specimens of each group. Statistical significance was determined using a student's t-test.

From: Yu X, Jia R, Huang J, Shu B, Zhu D, Liu Q, Gao X, Lin M, Yin Z, Wang M, Chen S, Wang Y, Chen X, Cheng A. Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge. Vet Res. 2012 Jul 6;43:56.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Duck CD8 Alpha Antibody, clone Du CD8-1 gallery image 3
Published customer image:
Mouse anti Duck CD8 antibody, clone Du CD8-1 (MCA2479) used for the evaluation of CD8 expression on Pekin duck lymphocytes by flow cytometry.
Image caption:
T lymphocytes in PBLs following DPV gC DNA vaccination. 3, 5, 7, 14, 28, 42 days after vaccination, the isolated PBLs were stained with monoclonal antibodies against duck CD4 (A), and CD8 (B). The results presented are the mean of all specimens of each group ± SD.

From: Lian B, Cheng A, Wang M, Zhu D, Luo Q, Jia R, Liu F, Han X, Chen X.
Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
Virol J. 2011 May 10;8:214.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Duck CD8 Alpha

Product Type
Monoclonal Antibody
Clone
Du CD8-1
Isotype
IgG2b
Specificity
CD8 Alpha

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Product Code Applications Pack Size List Price Your Price Qty
MCA2479
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP P 0.25 mg loader
List Price Your Price
loader

Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 recognizes Pekin duck CD8 alpha (CD8a). CD8a is expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells.

Most avian immunity research has been carried out on chickens and relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin.

Double-staining with clone Du CD8-1 and MCA2480 Mouse anti Duck IgY light chain clone 14A3 revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa (Kothlow et al. 1985). Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard (Anas platyrhynchos).

Target Species
Duck
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
293T cells expressing Pekin Duck CD8 alpha.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 10 ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)

References for CD8 Alpha antibody

  1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
    Dev Comp Immunol. 29 (8): 733-48.
  2. Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.
    Vet Res. 43: 56.
  3. Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
    Virol J. 8: 214.
  4. Chen, S. et al. (2015) Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose.
    Immunobiology. 220 (6): 753-61.
  5. Chen, S. et al. (2015) Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection.
    Poult Sci. 94 (1): 17-24.
  6. Chen, S. et al. (2016) Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
    Int J Mol Sci. 17(12):1990.
  7. Cornelissen, J.B. et al. (2013) Differences in highly pathogenic avian influenza viral pathogenesis and associated early inflammatory response in chickens and ducks.
    Avian Pathol. 42 (4): 347-64.
  8. Wu, Y. et al. (2019) Changes in the small intestine mucosal immune barrier in Muscovy ducklings infected with Muscovy duck reovirus
    Veterinary Microbiology. 233: 85-92.
    9. View The Latest Product References
  9. Apinda, N. et al. (2022) Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing Pasteurella multocida OmpH Gene.
    Vaccines (Basel). 10 (8)Aug 19 [Epub ahead of print].

Further Reading

  1. Higgins, D.A. & Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.
    J Immunol Methods. 106 (1): 135-45.

Synonyms
T-cell Surface Glycoprotein CD8 Alpha Chain
RRID
AB_609604

MCA2479

150735 158958 180112

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