CD8 Alpha antibody | Du CD8-1
Mouse anti Duck CD4 antibody, clone Du CD4-2 (MCA2478) used along with Mouse anti Duck CD8&aplha; antibody, clone Du CD8-1 (MCA2479) for the demonstration of lymphocyte subsets in goose spleen sections by immunohistochemistry using formalin fixed, paraffin embedded tissue sections.
Image caption:
Global characteristics of goslings after infection with GPV and H9N2. (A) Body weight change of GPV- and H9N2-infected goslings from day 0 to 5. ** p≤0.01, *** p≤0.001; (B) the expression level of IL-1β and IL-6 in the spleen tissues of GPV- and H9N2-infected goslings. ** p≤0.01, *** p≤0.001; (C) histological changes in goose spleens infected with GPV and H9N2 at 5 dpi. The spleen tissues from (a) mock-, (b) GPV-, and (c) H9N2-infected goslings were stained with H &E, and diffuse hemorrhage was observed in either of virus-infected spleen sections, scale bar = 100 μm; and (D) Detection of (a) GPV and (b) H9N2 antigen, as well as (c,d) CD4- and (e,f) CD8α-positive cells by immunohistochemical analysis. The dark brown represents positive signs for viral antigen, CD4, or CD8α molecules, scale bar = 100 μm.
From: Chen S, Wang A, Sun L, Liu F, Wang M, Jia R, Zhu D, Liu M, Yang Q, Wu Y, Sun K, Chen X, Cheng A.
Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
Int J Mol Sci. 2016 Dec 1;17(12). pii: E1990.
Mouse anti Duck CD8 antibody, clone Du CD8-1 (MCA2479) used for the evaluation of lymphocyte populations in PBLs following vaccination by flow cytometry.
Image caption:
T lymphocytes in PBL following DEV UL24 DNA vaccination. 1, 2, 3, 4, 6, 8 weeks after vaccination, the isolated PBL were stained with monoclonal antibodies against duck CD4 (A) and CD8 (B). The results presented are the mean of all specimens of each group. Statistical significance was determined using a student's t-test.
From: Yu X, Jia R, Huang J, Shu B, Zhu D, Liu Q, Gao X, Lin M, Yin Z, Wang M, Chen S, Wang Y, Chen X, Cheng A. Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge. Vet Res. 2012 Jul 6;43:56.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Duck CD8 antibody, clone Du CD8-1 (MCA2479) used for the evaluation of CD8 expression on Pekin duck lymphocytes by flow cytometry.
Image caption:
T lymphocytes in PBLs following DPV gC DNA vaccination. 3, 5, 7, 14, 28, 42 days after vaccination, the isolated PBLs were stained with monoclonal antibodies against duck CD4 (A), and CD8 (B). The results presented are the mean of all specimens of each group ± SD.
From: Lian B, Cheng A, Wang M, Zhu D, Luo Q, Jia R, Liu F, Han X, Chen X.
Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
Virol J. 2011 May 10;8:214.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 recognizes Pekin duck CD8 alpha (CD8a). CD8a is shown to be expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells.
Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA).
Double-staining with clone Du CD8-1 and MCA2480 Mouse anti Duck IgY light chain clone 14A3 revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa (Kothlow et al. 1985). Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard (Anas platyrhynchos).
Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA).
Double-staining with clone Du CD8-1 and MCA2480 Mouse anti Duck IgY light chain clone 14A3 revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa (Kothlow et al. 1985). Mouse anti Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard (Anas platyrhynchos).
Product Details
- Target Species
- Duck
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- 293T cells expressing Pekin Duck CD8 alpha.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD8 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1 | 10 ug/ml | |
| Immunohistology - Paraffin | |||
| Immunoprecipitation |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
Product Specific References
References for CD8 Alpha antibody
-
Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
Dev Comp Immunol. 29 (8): 733-48. -
Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.
Vet Res. 43: 56. -
Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.
Virol J. 8: 214. -
Chen, S. et al. (2015) Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose.
Immunobiology. 220 (6): 753-61. -
Chen, S. et al. (2015) Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection.
Poult Sci. 94 (1): 17-24. -
Chen, S. et al. (2016) Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
Int J Mol Sci. 17 (12): . -
Cornelissen, J.B. et al. (2013) Differences in highly pathogenic avian influenza viral pathogenesis and associated early inflammatory response in chickens and ducks.
Avian Pathol. 42 (4): 347-64. -
Wu,Y. et al. (2019) Changes in the small intestine mucosal immune barrier in Muscovy ducklings infected with Muscovy duck reovirus
Veterinary Microbiology. [Epub ahead of print].
Further Reading
-
Higgins, D.A. & Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.
J Immunol Methods. 106 (1): 135-45.
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