CD4/CD8 | YKIX302.9 / YCATE55.9

Rat anti Dog CD4:FITC/CD8:RPE

Product Type
Dual Color Reagent
Clone
YKIX302.9 / YCATE55.9
Isotype
Cocktail
Specificity
CD4/CD8

Product Code Applications Pack Size List Price Your Price Qty
DC048
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Canine CD4/CD8 antibodies, clones YKIX302.9 and YCATE55.9 is a dual colour reagent recognising canine CD4 and CD8 cell surface antigens. CloneYKIX302.9 recognises canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.

Clone YCATE55.9 recognises CD8α expressed by cytotoxic T cells.

Target Species
Dog
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG2a (RAT)
RPE reagent: IgG1 (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for CD4/CD8

  1. Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
    J Vet Med Sci. 68:1219-1221
  2. Mie, K. et al. (2016) Change in peripheral blood lymphocyte count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells combined with palliative tumor resection.
    Vet Immunol Immunopathol. 177: 58-63.
  3. Corsetti, S. et al. (2018) Bold personality makes domestic dogs entering a shelter less vulnerable to diseases.
    PLoS One. 13 (3): e0193794.
  4. Hassankhani, M. et al. (2017) The effects of prolonged azathioprine administration on blood cells, lymphocytes and immunoglobulins of Iranian mixed-breed dogs.
    Ir J Vet Med, 11(4), 361-77.

RRID
AB_324362
UniProt
P33705
P33706
Entrez Gene
CD4
CD8A
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0045058 T cell selection
View more products with CD4 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Dog Products