CD4/CD8 | YKIX302.9 / YCATE55.9
Rat anti Dog CD4:FITC/CD8:RPE
- Product Type
- Dual Color Reagent
- Clone
- YKIX302.9 / YCATE55.9
- Isotype
- Cocktail
- Specificity
- CD4/CD8
Antibody Quality and Performance Guarantee
| Rat anti Canine CD4/CD8 antibodies, clones YKIX302.9 and YCATE55.9 is a dual colour reagent recognising canine CD4 and CD8 cell surface antigens. CloneYKIX302.9 recognises canine CD4, which is expressed by lymphocyte subsets and canine neutrophils. Clone YCATE55.9 recognises CD8α expressed by cytotoxic T cells. |
- Target Species
- Dog
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent: IgG2a (RAT)
RPE reagent: IgG1 (RAT) - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD4/CD8
-
Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
J Vet Med Sci. 68:1219-1221 -
Mie, K. et al. (2016) Change in peripheral blood lymphocyte count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells combined with palliative tumor resection.
Vet Immunol Immunopathol. 177: 58-63. -
Corsetti, S. et al. (2018) Bold personality makes domestic dogs entering a shelter less vulnerable to diseases.
PLoS One. 13 (3): e0193794. -
Hassankhani, M. et al. (2017) The effects of prolonged azathioprine administration on blood cells, lymphocytes and immunoglobulins of Iranian mixed-breed dogs.
Ir J Vet Med, 11(4), 361-77.
- RRID
- AB_324362
- UniProt
- P33705
- P33706
- Entrez Gene
- CD4
- CD8A
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0045058 T cell selection
DC048
149650
161555
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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