CD4/CD8 | YKIX302.9 / YCATE55.9

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F ResetRat anti Dog CD4:FITC/CD8:RPE
- Product Type
- Dual Color Reagent
- Clone
- YKIX302.9 / YCATE55.9
- Isotype
- Cocktail
- Specificity
- CD4/CD8
Rat anti Canine CD4/CD8 antibodies, clones YKIX302.9 and YCATE55.9 is a dual colour reagent recognising canine CD4 and CD8 cell surface antigens. CloneYKIX302.9 recognises canine CD4, which is expressed by lymphocyte subsets and canine neutrophils. Clone YCATE55.9 recognises CD8α expressed by cytotoxic T cells. |
- Target Species
- Dog
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent: IgG2a (RAT)
RPE reagent: IgG1 (RAT) - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD4/CD8
-
Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
J Vet Med Sci. 68:1219-1221 -
Mie, K. et al. (2016) Change in peripheral blood lymphocyte count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells combined with palliative tumor resection.
Vet Immunol Immunopathol. 177: 58-63. -
Corsetti, S. et al. (2018) Bold personality makes domestic dogs entering a shelter less vulnerable to diseases.
PLoS One. 13 (3): e0193794. -
Hassankhani, M. et al. (2017) The effects of prolonged azathioprine administration on blood cells, lymphocytes and immunoglobulins of Iranian mixed-breed dogs.
Ir J Vet Med, 11(4), 361-77.
- RRID
- AB_324362
- UniProt
- P33705
- P33706
- Entrez Gene
- CD4
- CD8A
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0045058 T cell selection
DC048


If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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