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CD3/CD8 | CA17.2A12 / YCATE55.9

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anti Dog CD3:FITC/CD8:RPE, clone CA17.2A12 / YCATE55.9 thumbnail image 1
anti Dog CD3:FITC/CD8:RPE, clone CA17.2A12 / YCATE55.9 gallery image 1
FITC conjugated Mouse anti Canine CD3 and PE conjugated Rat anti Canine CD8 (DC047). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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anti Dog CD3:FITC/CD8:RPE

Product Type
Dual Color Reagent
Clone
CA17.2A12 / YCATE55.9
Isotype
Cocktail
Specificity
CD3/CD8

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Technical Suppport
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DC047
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Anti Canine CD3 / CD8 dual color reagent is a dual colour reagent recognising canine CD3 and CD8 cell surface antigens. Clone CA17.2A12 recognises canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes.

Clone YCATE 55.9 recognises canine CD8, which is expressed by cytotoxic T cells. YCATE 55.9 binds to CD8 alpha.

Target Species
Dog
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for CD3/CD8

  1. Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
    J Vet Med Sci. 68:1219-1221
  2. Karayannopoulou, M. et al. (2022) Effect of major versus minor mastectomy on host immunity in canine mammary cancer
    Vet Immunol Immunopathol. 24 Feb: 110403.

RRID
AB_324122
UniProt
P27597
P33706
Entrez Gene
CD3E
CD8A
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
View more products with CD3 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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