CD3/CD4 | CA17.2A12/YKIX302.9

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anti Dog CD3:FITC/CD4:RPE

Product Type
Dual Color Reagent
Clone
CA17.2A12/YKIX302.9
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC046 F 100 Tests
DC046 is a dual colour reagent recognising canine CD3 and CD4 cell surface antigens. Clone CA17.2A12 recognises canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes.

Clone YKIX 302.9 recognises canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.

Product Details

Target Species
Dog
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (RAT)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P27597 Related reagents
P33705 Related reagents
Entrez Gene
CD3E Related reagents
CD4 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007155 cell adhesion
GO:0006955 immune response
GO:0045058 T cell selection
Regulatory
For research purposes only

Applications of CD3/CD4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Product Specific References

References for CD3/CD4

  1. Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
    J Vet Med Sci. 68:1219-1221