CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9
Anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647 is a triple color reagent recognizing canine CD3, CD4 and CD8 cell surface antigens.
FITC conjugated Mouse anti Canine CD3 antibody, clone CA17.2A12 (MCA1477F) recognizes canine CD3, expressed by T lymphocytes. RPE conjugated Mouse anti Canine CD4 antibody, clone YKIX 302.9 (MCA1038PE) recognizes canine CD4, expressed by lymphocyte subsets and canine neutrophils. Alexa Fluor®647 conjugated Mouse anti Canine CD8 antibody, clone YCATE55.9 (MCA1039A647) recognizes CD8 alpha, expressed by cytotoxic T cells.
- Target Species
- Product Form
- Triple Color combination consisting of FITC, RPE, Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitute with 1.0 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
- Antibody Isotypes
- CD3 FITC reagent: Mouse IgG1CD4 RPE reagent: Rat IgG2aCD8 Alexa Fluor® 647 reagent: Rat IgG1
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007155 cell adhesion
- GO:0006955 immune response
- GO:0045058 T cell selection
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or firstname.lastname@example.org
- For research purposes only
Applications of CD3/CD4/CD8
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Negative Isotype Controls Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:Alexa Fluor® 647 -ve Control||TC023||F*||100 Tests/1ml|
Product Specific References
References for CD3/CD4/CD8
Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
J Vet Med Sci. 68:1219-1221
Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
PLoS One. 11 (5): e0154429.
Maina, E. et al. (2019) Food allergen-specific sublingual immunotherapy modulates peripheral T cell responses of dogs with adverse food reactions.
Vet Immunol Immunopathol. 212: 38-42.
How to Use the Spectraviewer?
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra