CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9

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anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647

Product Type
Triple Color Reagent
Clone
CA17.2A12 / YKIX302.9 / YCATE55.9
Isotype
Cocktail
Product Code Applications Pack Size List Price Quantity
TC014
Datasheet
F
MSDS
100 Tests/1ml loader

Anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647 is a triple color reagent recognizing canine CD3, CD4 and CD8 cell surface antigens.

FITC conjugated Mouse anti Canine CD3 antibody, clone CA17.2A12 (MCA1477F) recognizes canine CD3, expressed by T lymphocytes. RPE conjugated Mouse anti Canine CD4 antibody, clone YKIX 302.9 (MCA1038PE) recognizes canine CD4, expressed by lymphocyte subsets and canine neutrophils. Alexa Fluor®647 conjugated Mouse anti Canine CD8 antibody, clone YCATE55.9 (MCA1039A647) recognizes CD8 alpha, expressed by cytotoxic T cells.

Product Details

Target Species
Dog
Product Form
Triple Color combination consisting of FITC, RPE, Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
CD3 FITC reagent: Mouse IgG1
CD4 RPE reagent: Rat IgG2a
CD8 Alexa Fluor® 647 reagent: Rat IgG1

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of reconstitution

More Information

UniProt
P27597 Related reagents
P33705 Related reagents
P33706 Related reagents
Entrez Gene
CD3E Related reagents
CD4 Related reagents
CD8A Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007155 cell adhesion
GO:0006955 immune response
GO:0045058 T cell selection
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD3/CD4/CD8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:Alexa Fluor® 647 -ve Control TC023 F* 100 Tests/1ml loader

Product Specific References

References for CD3/CD4/CD8

  1. Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
    J Vet Med Sci. 68:1219-1221
  2. Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
    PLoS One. 11 (5): e0154429.
  3. Maina, E. et al. (2019) Food allergen-specific sublingual immunotherapy modulates peripheral T cell responses of dogs with adverse food reactions.
    Vet Immunol Immunopathol. 212: 38-42.