TNF Alpha antibody | CC328

100% Secure

Mouse anti Bovine TNF Alpha:Biotin

Product Type
Monoclonal Antibody
Clone
CC328
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2335B E 0.25 mg
Mouse anti Bovine TNF alpha antibody, clone CC328 recognizes bovine TNF alpha, a 17.5kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.

The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant bovine TNF alpha.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q06599 Related reagents
Entrez Gene
TNF Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
Biotinylated Mouse anti Bovine TNF alpha antibody, clone CC328 may be used as a detection antibody in a sandwich ELISA for bovine TNF alpha in combination with Mouse anti Bovine TNFα antibody, clone CC327 (MCA2334) as capture reagent. Recombinant Bovine TNFα (PBP005) may be used as a standard.

Application Based External Images

ELISA

Product Specific References

References for TNF Alpha antibody

  1. Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.
    Vet. Immunol. Immunopathol. 95: 21-31.
  2. Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide.
    Infect Immun. 71 (11): 6420-5.
  3. Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells.
    Cell Microbiol. 5 (10): 709-16.
  4. Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumour necrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.
    Vet Immunol Immunopathol. 135 (3-4): 320-4.
  5. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
    Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
  6. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.
  7. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  8. Camejo, M.I. et al. (2014) TNF-alpha in bulls experimentally infected with Trypanosoma vivax: a pilot study.
    Vet Immunol Immunopathol. 162 (3-4): 192-7.