MHC Class II DQ antibody | CC158

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Mouse anti Bovine MHC Class II DQ:RPE

Product Type: Monoclonal Antibody
Clone: CC158
Isotype: IgG2a
Specificity: MHC Class II DQ

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References (5)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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MCA5655PE Datasheet SDS SDS	F	100 Tests		Log in
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Anti Bovine MHC Class II DQ Antibody, clone CC158 thumbnail image 1

Anti Bovine MHC Class II DQ Antibody, clone CC158 thumbnail image 2

Anti Bovine MHC Class II DQ Antibody, clone CC158 thumbnail image 3

Anti Bovine MHC Class II DQ Antibody, clone CC158 thumbnail image 4

Anti Bovine MHC Class II DQ Antibody, clone CC158 gallery image 1 — Bovine peripheral blood lymphocytes stained with Mouse anti Bovine MHC Class II DQ antibody, clone CC158 (**MCA5655**) followed by FITC conjugated Goat anti Mouse IgG (STAR117F) analyzed by flow cytometry.

Anti Bovine MHC Class II DQ Antibody, clone CC158 gallery image 2 — Bovine peripheral lymphocytes stained with FITC conjugated Mouse anti Bovine MHC Class II DQ antibody, clone CC158 (**MCA5655F**) analyzed by flow cytometry.

Anti Bovine MHC Class II DQ Antibody, clone CC158 gallery image 3 — Bovine peripheral blood lymphocytes stained with RPE conjugated Mouse anti Bovine MHC Class II DQ antibody, clone CC158 (**MCA5655PE**) analyzed by flow cytometry.

Anti Bovine MHC Class II DQ Antibody, clone CC158 gallery image 4 — **Published customer image:**
Mouse anti Bovine MHC class II DQ antibody, clone CC158 (**MCA5655**) used to evaluate MHC Class II expression on *Salmonella* infected bovine cells by flow cytometry.
**Image caption:**
Identification and characterisation of *Salmonella*-infected bovine cells. Single cell suspensions were prepared from the ileum and LNs of the three calves challenged orally with SD3246-GFP. B The majority of SD3246-GFP-infected cells also expressed CD40, CD80, CD86, CD11b and CD11c but lacked CD1 expression suggesting that they were macrophages. The data shown here are from mesenteric lymph nodes of an infected calf and are representative of infected ileal mucosa and LNs.

From: Vohra P, Vrettou C, Hope JC, Hopkins J, Stevens MP.
Nature and consequences of interactions between *Salmonella enterica* serovar Dublin and host cells in cattle.
**Vet Res. 2019 Nov 27;50(1):99**.
doi: 10.1186/s13567-019-0720-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Bovine MHC class II DQ antibody, clone CC158 recognizes Bovine MHC Class II DQ. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA gene cluster, DR and DQ each composed of an alpha and beta chain.

Target Species: Bovine
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1.0ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

RPE 488nm laser 496 578
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature.
Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul. .

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Description	Mouse IgG2a Negative Control:RPE

References for MHC Class II DQ antibody

Stephens, S.A. & Howard, C.J. (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
Parasitology. 124 (Pt 5): 485-93.
Van Rhijn, I. et al. (2007) Massive, sustained gammadelta T cell migration from the bovine skin in vivo.
J Leukoc Biol. 81: 968-73.
Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.
J Leukoc Biol. 83 (6): 1396-403.
Price,S.J. and Hope, J.C. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.
Immunology. 126: 201-8.
Vohra, P. et al. (2019) Nature and consequences of interactions between Salmonella enterica serovar Dublin and host cells in cattle.
Vet Res. 50 (1): 99.