IL-2 antibody | AbD22297
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E ResetHuman anti Bovine Interleukin-2
- Product Type
- Monoclonal Antibody
- Clone
- AbD22297
- Isotype
- HuCAL Fab bivalent
- Specificity
- IL-2
Antibody Quality and Performance Guarantee
| Human anti Bovine Interleukin-2, clone AbD22297 recognizes bovine interleukin-2 (IL-2), also known as T-cell growth factor (TCGF). Bovine IL-2 is a 155 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation. Human anti bovine interleukin-2, clone AbD22297, has been successfully used for the detection and measurement of native and recombinant bovine IL-2 in ELISA where it acts as the detection antibody in a sensitive assay for bovine IL-2 determination. Bio-Rad recommend clone AbD21766 (HCA267) for intracellular flow cytometry. |
- Target Species
- Bovine
- Product Form
- A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a V5 tag and a double extended Strep-tag (SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK).
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Fc-fusion protein containing the amino acid sequence 21-155 from bovine interleukin-2
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=0.3 nM as measured by real time, label-free molecular interaction analysis on recombinant bovine IL-2.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| ELISpot | |
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| Flow Cytometry |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA together with HCA267 as the capture reagent.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti V5-Tag:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human anti Bovine Interleukin-2 | HCA267 | E ES F * | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human anti Bovine Interleukin-2 | ||||||
| Streptavidin:HRP | STAR5B | C E P WB | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Streptavidin:HRP | ||||||
Further Reading
-
Rhodes, S.G. et al. (2014) Use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with Mycobacterium bovis BCG and cattle infected with M. bovis.
Clin Vaccine Immunol. 21:39-45.
- UniProt
- P05016
- Entrez Gene
- IL2
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA268
1604
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