IL-12 / IL-23 antibody | CC326

Mouse anti Bovine Interleukin-12/23:Biotin

Product Type
Monoclonal Antibody
Clone
CC326
Isotype
IgG2b
Specificity
IL-12 / IL-23

Product Code Applications Pack Size List Price Your Price Qty
MCA2173B
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Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 recognizes the p40 subunit of bovine interleukin-12. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A. Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 has been shown to block the biological activity of bovine IL-12.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant bovine IL-12.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells of 100ul.
ELISA
This biotin conjugate may be used as detection reagent in a sandwich ELISA assay using MCA1782EL as capture reagent, see Bannerman, D.D.et al.

References for IL-12 / IL-23 antibody

  1. Hope, J.C. et al. (2002) Development of detection methods for ruminant interleukin (IL)-12.
    J Immunol Methods. 266 (1-2): 117-26.
  2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  4. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  5. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
    J Immunol. 185: 3313-25.
  6. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  7. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  8. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
    J Virol. 82: 1777-86.
  9. View The Latest Product References
  10. Stephens, S.A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
    Immunology. 110: 48-57.
  11. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
    BMC Vet Res.10: 293.
  12. Beechler, B.R. et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).
    Proc Biol Sci. 282 (1805) pii: 20142942.
  13. Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.
    Biol Reprod. 95 (2): 39.
  14. Stabel, J.R. & Bannantine, J.P. (2021) Reduced tissue colonization of Mycobacterium avium. subsp. paratuberculosis. in neonatal calves vaccinated with a cocktail of recombinant proteins.
    Vaccine. May 06 [Epub ahead of print].
  15. Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
    Cytometry A. 91 (9): 901-7.
  16. Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
    Vet Immunol Immunopathol. 237: 110268.
  17. Ciliberti, M.G. et al. (2020) Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
    Front Vet Sci. 7: 9.

ELISA

Flow Cytometry

RRID
AB_324111
UniProt
P46282
P54349
Entrez Gene
IL12B
IL12A
GO Terms
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0030101 natural killer cell activation
GO:0042093 T-helper cell differentiation
GO:0042095 interferon-gamma biosynthetic process
GO:0042104 positive regulation of activated T cell proliferation
GO:0008083 growth factor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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