IL-12 / IL-23 antibody | CC326
Image caption:
IL-12 response (u/ml) in mesenteric lymph nodes against GRA7, TLA, MIC3 and EC2 in function of time.
From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E.
Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
IL-12 responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 μg/ml polyI:C, 0.5 &mug/ml R-848 or 5 μg/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) secretion. Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p≤0.01; **p≤0.005; ***p≤0.001; ****p≤0.0005.
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
MLN and spleen cell IL-12 responses to R-848 stimulation as a function of age. MLN and spleen cells from 6- to 14-day-old neonates (closed circles), 20-day-old lambs (closed diamonds) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 μg/ml R-848. At the end of the culture period, supernatants were harvested and ELISA carried out to assess IL-12 secretion by MLN cells (A) or spleen cells (B). Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates, 20-day-old lambs and adults: *p≤0.01; **p≤0.005; ***p≤0.001; ****p≤0.0005.
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Roles of TGFβ1 and IL-10 in regulating IL-12 responses to R-848. MLN cells from neonates (closed circles) and adults (open squares) were cultured in vitro for 48 h, with or without 0.5 μg/ml R-848, in the presence of rhTGFβ1 (n = 7) (A) or rovIL-10 (n = 5) (B). Supernatants were harvested and ELISA was carried out to assess IL-12 secretion. The mean ± SEM level of IL-12 secretion is shown (A, B). RNA was extracted and purified from freshly isolated MLN cells. IL-10 mRNA levels were determined by quantitative RT-PCR. Median normalized values are presented for neonates (closed circles) and adults (open squares) (C). MLN cells from neonates (closed circles) and adults (open squares) were stimulated in vitro for 48 h with or without 0.5 μg/ml R-848. Supernatants were harvested and ELISA carried out to assess IL-10 secretion. Medians are indicated. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: **p≤0.001 (D). RNA was extracted and purified from freshly isolated MLN cells. Foxp3 mRNA levels were determined by quantitative RT-PCR. Median normalised values are presented for neonates (closed circles) and adults (open squares) (E). Comparison of the IL-12 response of neonate MLN cells to R-848 stimulation in culture medium supplemented with 10%FCS, 10% neonate autologous plasma or 10% adult plasma. Paired t-test between neonate and adult plasma were non-significant (F).
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Main cell population producing IL-12 in response to R-848. Neonatal MLN cells were cultured in vitro with or without 0.5 μg/ml R-848 and, at various time points, supernatants were harvested and ELISA was carried out for IL-12. Mean ± SEM IL-12 secretion is shown for each time point (A). Neonatal MLN cells were cultured in vitro with (black bars) or without (hatched bars) 0.5 μg/ml R-848 for 8 h, with brefeldin A added for the last 5 h. Cells were harvested for IL-12 intracellular staining combined with labelling of CD11b, CD14, MHC class II and CD205. Three independent experiments were carried out and the data shown are the mean proportions of IL-12+ cells after gating on CD11b+, CD14+, MHC class II+ or CD205+ MLN cells (B).
From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010)
Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 (MCA1782EL) as a capture reagent in a sandwich ELISA with biotinylated Mouse anti Bovine IL-12/23 antibody, clone CC326 (MCA2173B) as a detection reagent for the measurement of IL-12 secretion by bovine PBMC.
Image caption:
Interleukin-12 secretion by PBMCs unstimulated (UNC) and stimulated with LPS and ConA (SC, 1 and 5 μg/ml, respectively) and with 4 mM of H2O2 for 24 h at 37°C/5% CO2. *P <0.05 were considered significant among feeding strategy.
From: Ciliberti MG, Albenzio M, De Palo P, Santillo A, Caroprese M.
Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
Front Vet Sci. 2020 Feb 20;7:9.
doi: 10.3389/fvets.2020.00009.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 recognizes the p40 subunit of bovine interleukin-12. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A. Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 has been shown to block the biological activity of bovine IL-12.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant bovine IL-12.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46282
- P54349
- Entrez Gene
- IL12B
- IL12A
- GO Terms
- GO:0016020 membrane
- GO:0004896 cytokine receptor activity
- GO:0005125 cytokine activity
- GO:0005143 interleukin-12 receptor binding
- GO:0030101 natural killer cell activation
- GO:0042093 T-helper cell differentiation
- GO:0042095 interferon-gamma biosynthetic process
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0008083 growth factor activity
- Regulatory
- For research purposes only
Applications of IL-12 / IL-23 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/1000 | |
| Flow Cytometry 1 | 1/1000 | ||
| Functional Assays | 1/100 | ||
| ELISA | 5ug/ml |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells of 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells of 100ul.
- ELISA
- This biotin conjugate may be used as detection reagent in a sandwich ELISA assay using MCA1782EL as capture reagent, see Bannerman, D.D.et al.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:Preservative Free | MCA691XZ | C F P R RE | 1 mg |
|
Product Specific References
References for IL-12 / IL-23 antibody
-
Hope, J.C. et al. (2002) Development of detection methods for ruminant interleukin (IL)-12.
J Immunol Methods. 266 (1-2): 117-26. -
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. -
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38. -
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. -
Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. -
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72. -
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. -
Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
J Virol. 82: 1777-86. -
Stephens, S.A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
Immunology. 110: 48-57. -
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res.10: 293. -
Beechler, B.R. et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).
Proc Biol Sci. 282 (1805) pii: 20142942. -
Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.
Biol Reprod. 95 (2): 39. -
Stabel, J.R. & Bannantine, J.P. (2021) Reduced tissue colonization of Mycobacterium avium. subsp. paratuberculosis. in neonatal calves vaccinated with a cocktail of recombinant proteins.
Vaccine. May 06 [Epub ahead of print]. -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7. -
Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
Vet Immunol Immunopathol. 237: 110268. -
Ciliberti, M.G. et al. (2020) Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
Front Vet Sci. 7: 9.
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