IL-12 / IL-23 antibody | CC326





Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant bovine IL-12.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46282
- P54349
- Entrez Gene
- IL12B
- IL12A
- GO Terms
- GO:0016020 membrane
- GO:0004896 cytokine receptor activity
- GO:0005125 cytokine activity
- GO:0005143 interleukin-12 receptor binding
- GO:0030101 natural killer cell activation
- GO:0042093 T-helper cell differentiation
- GO:0042095 interferon-gamma biosynthetic process
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0008083 growth factor activity
- Regulatory
- For research purposes only
Applications of IL-12 / IL-23 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/100 | 1/1000 | |
Flow Cytometry 1 | 1/1000 | ||
Functional Assays | 1/100 | ||
ELISA | 5ug/ml |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells of 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells of 100ul.
- ELISA
- This biotin conjugate may be used as detection reagent in a sandwich ELISA assay using MCA1782EL as capture reagent, see Bannerman, D.D.et al.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2b Negative Control:Preservative Free | MCA691XZ | C F P R RE | 1 mg |
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Product Specific References
References for IL-12 / IL-23 antibody
-
Hope, J.C. et al. (2002) Development of detection methods for ruminant interleukin (IL)-12.
J Immunol Methods. 266 (1-2): 117-26. -
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. -
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38. -
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. -
Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. -
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72. -
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. -
Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
J Virol. 82: 1777-86. -
Stephens, S.A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
Immunology. 110: 48-57. -
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res.10: 293. -
Beechler, B.R. et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).
Proc Biol Sci. 282 (1805) pii: 20142942. -
Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.
Biol Reprod. 95 (2): 39.
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