CD45 antibody | CC1
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Mouse anti Bovine CD45:FITC
- Product Type
- Monoclonal Antibody
|Mouse anti Bovine CD45 antibody, clone CC1 recognizes the bovine homologue of the human CD45 antigen, also known as leucocyte common antigen (LCA),
CD45 is a pan leucocyte cell surface marker expressed on all cells of hematopoietic origin except for erythrocytes (Bembridge et al. 1993). CD45 occurs in a number of isoforms, which in bovine includes CD45R, CD45RA, CD45RB and CD45RO and which have restricted cellular expression.
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Bovine thoracic duct lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse IgG1 Negative Control:FITC||MCA928F||F||100 Tests||Log in|
|List Price||Your Price|
|Description||Mouse IgG1 Negative Control:FITC|
References for CD45 antibody
Bembridge, G.P. et al. (1993) Identification of monoclonal antibodies specific for bovine leukocyte common antigen (CD45) together with a novel broadly expressed leukocyte differentiation antigen, BoWC11.
Vet Immunol Immunopathol. 39: 115-20.
Inchaisri, C. et al. (2000) Studies on the modulation of leucocyte subpopulations and immunoglobulins following intramammary infusion of beta 1,3-glucan into the bovine udder during the dry period.
J Vet Med B Infect Dis Vet Public Health. 47 (5): 373-86.
Hu, S. et al. (2001) Effect of subcutaneous injection of ginseng on cows with subclinical Staphylococcus aureus mastitis.
J Vet Med B Infect Dis Vet Public Health. 48 (7): 519-28.
Gånheim, C. et al. (2005) Changes in peripheral blood leucocyte counts and subpopulations after experimental infection with BVDV and/or Mannheimia haemolytica.
J Vet Med B Infect Dis Vet Public Health. 52 (9): 380-5.
Niku, M. et al. (2006) Identification of major cell types in paraffin sections of bovine tissues.
BMC Vet Res. 2: 5.
Grönlund, U. et al. (2006) Changes in blood and milk lymphocyte sub-populations during acute and chronic phases of Staphylococcus aureus induced bovine mastitis.
Res Vet Sci. 80 (2): 147-54.
Galatowicz, G. et al. (2007) Ocular anti-allergic compounds selectively inhibit human mast cell cytokines in vitro and conjunctival cell infiltration in vivo.
Clin Exp Allergy. 37 (11): 1648-56.
Riondato, F. et al. (2008) Effects of road transportation on lymphocyte subsets in calves.
Vet J. 175 (3): 364-8.
View The Latest Product References
Huang, L. et al. (2010) A hierarchy of endothelial colony-forming cell activity displayed by bovine corneal endothelial cells.
Invest Ophthalmol Vis Sci. 51: 3943-9.
Spalenza, V. et al. (2011) Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes.
Vet J. 189 (3): 278-83.
Lynch, E.M. et al. (2012) Effect of pre-weaning concentrate supplementation on peripheral distribution of leukocytes, functional activity of neutrophils, acute phase protein and behavioural responses of abruptly weaned and housed beef calves.
BMC Veterinary Research 8: 1
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441.
Lee, J. et al. (2020) Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.
Biosci Rep. 40 (4): BSR20181829.
Ballingall, K.T. et al. (2001) The CD45 locus in cattle: allelic polymorphism and evidence for exceptional positive natural selection.
Immunogenetics. 52: 276-83.
MCA832F158341 160952 1807
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Bovine Products
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