CD44 antibody | IL-A118
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|Mouse anti Bovine CD44 antibody, clone IL-A118 recognizes bocine Phagocytic Glycoprotein-1 (PGP-1), also known as CD44, Hermes antigen, Extracellular matrix receptor III or HUTCH-1. Bovine CD44 is a 346 amino acid ~90 kDa type I single pass transmembrane glycoprotein containing a single Link domain, responsible for hyaluronan binding. Bovine CD44 is expressed by a wide range of bovine cells, including peripheral T and B lymphocytes, monocytes, granulocytes, platelets and early erythroid cells.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Camel
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.853 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD44 antibody
Naessens, J. & Nthale, J. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies.
Vet Immunol Immunopathol. 39 (1-3): 217-23.
Menge C et al. (2004) Bovine ileal intraepithelial lymphocytes represent target cells for Shiga toxin 1 from Escherichia coli.
Infect Immun. 72 (4): 1896-905.
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90.
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47.
de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
Cell Biol Int. 40 (12): 1332-9.
de Moraes, C.N. et al. (2017) Shotgun proteomic analysis of the secretome of bovine endometrial mesenchymal progenitor/stem cells challenged or not with bacterial lipopolysaccharide.
Vet Immunol Immunopathol. Mar 27 [Epub ahead of print]
Lee, J. et al. (2020) Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.
Biosci Rep. 40 (4): BSR20181829.
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