CD44 antibody | IL-A118

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Mouse anti Bovine CD44:FITC

Product Type
Monoclonal Antibody
Clone
IL-A118
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2433F F 0.1 mg
Mouse anti Bovine CD44 antibody, clone IL-A118 recognizes bocine Phagocytic Glycoprotein-1 (PGP-1), also known as CD44, Hermes antigen, Extracellular matrix receptor III or HUTCH-1. Bovine CD44 is a 346 amino acid ~90 kDa type I single pass transmembrane glycoprotein containing a single Link domain, responsible for hyaluronan binding. Bovine CD44 is expressed by a wide range of bovine cells, including peripheral T and B lymphocytes, monocytes, granulocytes, platelets and early erythroid cells.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Camel
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.853 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q29423 Related reagents
Entrez Gene
CD44 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005540 hyaluronic acid binding
Regulatory
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Product Specific References

References for CD44 antibody

  1. Naessens, J. & Nthale, J. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies.
    Vet Immunol Immunopathol. 39 (1-3): 217-23.
  2. Menge C et al. (2004) Bovine ileal intraepithelial lymphocytes represent target cells for Shiga toxin 1 from Escherichia coli.
    Infect Immun. 72 (4): 1896-905.
  3. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
    Vet Immunol Immunopathol. 39 (1-3): 283-90.
  4. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  5. de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
    Cell Biol Int. 40 (12): 1332-9.
  6. de Moraes, C.N. et al. (2017) Shotgun proteomic analysis of the secretome of bovine endometrial mesenchymal progenitor/stem cells challenged or not with bacterial lipopolysaccharide.
    Vet Immunol Immunopathol. Mar 27 [Epub ahead of print]