CD44 antibody | IL-A118
Phycoerythrin conjugated Mouse anti Bovine CD44 antibody, clone IL-A118 (MCA2433PE) used to assess CD44 on bovine mesenchymal stem cells by flow cytometry. Shown with a negative marker of bovine MSCs, CD45 using FITC conjugated Mouse anti Bovine CD45 (MCA832F).
Image caption:
Cell surface markers of boT-MSCs.
Cell surface markers (positive: CD44, CD90 negative: CD45) observed (P3) by flow cytometry analysis. Data are expressed as means ± standard error of values, and were obtained by three determinations.
From: Lee J, Byeon JS, Gu NY, Lee S, Lee SA, Jeong DU, Ouh IO, Cho IS, Song JY, Lee YH, Hyun BH.
Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.
Biosci Rep. 2020 Apr 30;40(4):BSR20181829.
doi: 10.1042/BSR20181829.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Bovine CD44 antibody, clone IL-A118 (MCA2433F) used to label CD44 expressing bovine nucleus pulposus cells by immunofluorescence.
Image caption:
(B) CD44 immunofluorescence staining after 2 days of culture. Representative images of sagittal sections of IVD explants (scale bar: 50 μm) Quantification of the percentage of cells positive for CD44 was performed according with the scheme presented.
From:Ferreira JR, Caldeira J, Sousa M, Barbosa MA, Lamghari M, Almeida-Porada G, Gonçalves RM.
Dynamics of CD44+ bovine nucleus pulposus cells with inflammation.
Sci Rep. 2024 Apr 21;14(1):9156.
doi: 10.1038/s41598-024-59504-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD44:FITC
- Product Type
- Monoclonal Antibody
- Clone
- IL-A118
- Isotype
- IgG1
- Specificity
- CD44
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD44 antibody, clone IL-A118 recognizes bocine Phagocytic Glycoprotein-1 (PGP-1), also known as CD44, Hermes antigen, Extracellular matrix receptor III or HUTCH-1. Bovine CD44 is a 346 amino acid ~90 kDa type I single pass transmembrane glycoprotein containing a single Link domain, responsible for hyaluronan binding. Bovine CD44 is expressed by a wide range of bovine cells, including peripheral T and B lymphocytes, monocytes, granulocytes, platelets and early erythroid cells. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Camel - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.853 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for CD44 antibody
-
Naessens, J. & Nthale, J. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies.
Vet Immunol Immunopathol. 39 (1-3): 217-23. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Menge C et al. (2004) Bovine ileal intraepithelial lymphocytes represent target cells for Shiga toxin 1 from Escherichia coli.
Infect Immun. 72 (4): 1896-905. -
de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
Cell Biol Int. 40 (12): 1332-9. -
de Moraes, C.N.et al. (2017) Shotgun proteomic analysis of the secretome of bovine endometrial mesenchymal progenitor/stem cells challenged or not with bacterial lipopolysaccharide.
Vet Immunol Immunopathol. 187: 42-7. -
Lee, J. et al. (2020) Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.
Biosci Rep. 40 (4): BSR20181829. -
Molinos, M. et al. (2023) Alterations of bovine nucleus pulposus cells with aging.
Aging Cell. 22 (8): e13873.
View The Latest Product References
-
Ferreira, J.R. et al. (2024) Dynamics of CD44(+) bovine nucleus pulposus cells with inflammation.
Sci Rep. 14 (1): 9156. -
de Barcelos, M.S. et al. (2024) Extracellular vesicles derived from bovine adipose-derived mesenchymal stromal cells enhance in vitro embryo production from lesioned ovaries
Cytotherapy. 20 May [Epub ahead of print].
- Synonyms
- H-CAM
- RRID
- AB_1604795
- UniProt
- Q29423
- Entrez Gene
- CD44
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005540 hyaluronic acid binding
MCA2433F
150615
158799
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