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CD25 antibody | IL-A111

Mouse anti Bovine CD25:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price

Mouse anti Bovine CD25 antibody, clone IL-A111 recognizes the bovine CD25 cell surface antigen, a ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is expressed by activated T cells.

Mouse anti Bovine CD25 antibody, clone IL-A111 is reported to block the IL-2 driven proliferation of Con A-induced blast cells/ bovine lymphocytes (Naessens et al. 1992).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
Description Mouse IgG1 Negative Control:FITC

References for CD25 antibody

  1. Naessens, J. et al. (1992) Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene.
    Immunology. 76 (2): 305-9.
  2. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  3. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
    J Immunol. 159 (11): 5372-82.
  4. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
    Vet Immunol Immunopathol. 39 (1-3): 283-90.
  5. Evans, C.W. et al. (1994) Antigen recognition and activation of ovine gamma delta T cells.
    Immunology. 82 (2): 229-37.
  6. Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
    J Leukoc Biol. 63 (5): 567-74.
  7. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  8. Menge, C. et al. (2004) Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection.
    J Med Microbiol. 53: 573-9.
  9. View The Latest Product References
  10. Rhodes, S.G. et al. (1999) Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle.
    J Gen Virol. 80 : 1673-9.
  11. Piper, E.K. et al. (2009) Immunological profiles of Bos taurus and Bos indicus cattle infested with the cattle tick, Rhipicephalus (Boophilus) microplus.
    Clin Vaccine Immunol. 16: 1074-86.
  12. Woolums, A.R. et al. (2013) Effect of calf age and administration route of initial multivalent modified-live virus vaccine on humoral and cell-mediated immune responses following subsequent administration of a booster vaccination at weaning in beef calves.
    Am J Vet Res. 74: 343-54.
  13. Mcinnes, E. et al. (1999) Phenotypic analysis of local cellular responses in calves infected with bovine respiratory syncytial virus.
    Immunology. 96 (3): 396-403.
  14. Maślanka, T. et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
    Pol J Vet Sci. 15: 11-20.
  15. Menge, C. et al. (2003) Verotoxin 1 from Escherichia coli affects Gb3/CD77+ bovine lymphocytes independent of interleukin-2, tumor necrosis factor-alpha, and interferon-alpha.
    Exp Biol Med (Maywood). 228: 377-86.
  16. Menge, C. et al. (1999) Shiga toxin 1 from Escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro.
    Infect Immun. 67: 2209-17.
  17. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
    Int J Parasitol. 40: 865-75.
  18. Maślanska, T. and Jaroszewski, J.J. (2013) In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25-CD4+ cells.
    J Vet Sci. 14 (2): 125-34.
  19. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
    Theriogenology. 82 (3): 403-10.
  20. Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
    Dev Comp Immunol. 47 (2): 254-63.
  21. Kang, H.J. et al. (2016) Effects of Ambient Temperature on Growth Performance, Blood Metabolites, and Immune Cell Populations in Korean Cattle Steers.
    Asian-Australas J Anim Sci. 29 (3): 436-43.
  22. Schmidt, N. et al. (2018) Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.
    Vet Res. 49 (1): 28.
  23. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
    Vet Immunol Immunopathol. 202: 74-84.
  24. Brodzki, P. et al. (2020) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. 91 (1): e13306.
  25. Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
    Comp Immunol Microbiol Infect Dis. 68: 101388.
  26. de Araújo, F.F. et al. (2019) Distinct immune response profile during Rhipicephalus (Boophilus) microplus. infestations of guzerat dairy herd according to the maternal lineage ancestry (mitochondrial DNA).
    Vet Parasitol. 273: 36-44.
  27. Colombatti, M.O. et al. (2021) Evaluation of a virulent strain of Mycobacterium avium subsp. paratuberculosis used as a heat-killed vaccine.
    Vaccine. 39 (51): 7401-12.
  28. Tucker, N. et al. (2023) Bovine blood and milk T-cell subsets in distinct states of activation and differentiation during subclinical Staphylococcus aureus mastitis.
    J Reprod Immunol. 156: 103826.
  29. Blanco, F.C. et al. (2021) Identifying Bacterial and Host Factors Involved in the Interaction of Mycobacterium bovis with the Bovine Innate Immune Cells.
    Front Immunol. 12: 674643.
  30. Seemann, L. et al. (2024) Dietary L-carnitine supplementation modifies blood parameters of mid-lactating dairy cows during standardized lipopolysaccharide-induced inflammation.
    Front Immunol. 15: 1390137.

Flow Cytometry

IL-2R Alpha Chain
Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity


1610 166331 1711

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