CD21 antibody | CC51
Mouse anti Bovine CD21:RPE
- Product Type
- Monoclonal Antibody
- Clone
- CC51
- Isotype
- IgG2b
- Specificity
- CD21
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessens et al. 1990). CD21 may be expressed on B-cells as either a long or a short form (Pringle et al. 2012). Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig African Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- Bovine (Friesian cattle) mesenteric lymph node cells.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG2b Negative Control:RPE | ||||||
Source Reference
-
Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes.
Immunology. 69 (4): 525-30.
References for CD21 antibody
-
Denham S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.
Vet Immunol Immunopathol. 43: 259-67. -
Boersma W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
Vet Immunol Immunopathol. 80: 63-78. -
Tenaya I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.
Vet Immunol Immunopathol. 149: 167-76. -
Sinkora, M et al. (2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.
Dev Comp Immunol. 39: 409-18. -
Sinkora, M. et al. (2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.
Vet Res. 45: 91. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Li, J. et al. (2023) Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells.
Genes Genomics. Nov 18 [Epub ahead of print].
- Synonyms
- CR2
- UniProt
- Q8HY44
MCA5953PE
148617
168045
INN1707
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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