Growth Cone antibody | 2G13
Mouse anti Growth Cone antibody, clone 2G13 recognizes a protein originally termed 2G13P which is localized to growth cones. Subsequent investigation has identified this protein to be 40S ribosomal protein SA, also known as 37 kDa laminin receptor precursor or Laminin receptor 1 (Baloui et al. 2004). 40S ribosomal protein SA is a 296 amino acid ~37 kDa membrane, cytoplasmic and nuclear protein required for the assembly and/or stability of the 40S ribosomal subunit.. In vertebrate evolution the molecule has acquired a secondary function as a laminin receptor (UniProt: P50890). In growth cones expression is notable particularly in filopodia and lamellipodia in developing rat CNS and embryonic neurons in culture (Stettler et al. 1999).
40S ribosomal protein SA interacts with the filamentous actin cytoskeleton and therefore may be involved in growth cone motility (Stettler et al. 1999). Mouse anti Growth Cone antibody, clone 2G13 has been used for the detection of growth cones by immunohistochemistry and identification of 40S ribosomal protein SA by western blotting in chicken and rat samples (Baloui et al. 2004).
40S ribosomal protein SA interacts with the filamentous actin cytoskeleton and therefore may be involved in growth cone motility (Stettler et al. 1999). Mouse anti Growth Cone antibody, clone 2G13 has been used for the detection of growth cones by immunohistochemistry and identification of 40S ribosomal protein SA by western blotting in chicken and rat samples (Baloui et al. 2004).
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Embryonic chick tectal membranes.
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P50890 Related reagents
- Entrez Gene
- RPSA Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0003735 structural constituent of ribosome
- GO:0004872 receptor activity
- GO:0005634 nucleus
- GO:0006412 translation
- GO:0015935 small ribosomal subunit
- GO:0043236 laminin binding
- Regulatory
- For research purposes only
Applications of Growth Cone antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Clone 2G13 has also been reported to work in Western Blotting.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | 0.1 mg | F | ||
| Human anti Mouse IgM:HRP | HCA040P | 0.1 mg | E | ||
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | 1 ml | C E P WB | ||
| Goat anti Mouse IgM:RPE | STAR86PE | 0.5 ml | F | ||
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | 1 mg | C E WB | ||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | 1 mg | E |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Chicken CD184 / CXCR4 | MCA6012GA | 0.1 mg | C F IF |
Product Specific References
References for Growth Cone antibody
-
Stettler, O. et al. (1999) Monoclonal antibody 2G13, a new axonal growth cone marker.
J Neurocytol. 28 (12): 1035-44. -
Baloui, H. et al. (2004) Cellular prion protein/laminin receptor: distribution in adult central nervous system and characterization of an isoform associated with a subtype of cortical neurons.
Eur J Neurosci. 20 (10): 2605-16. -
Espejo, C. et al. (2005) Time-course expression of CNS inflammatory, neurodegenerative tissue repair markers and metallothioneins during experimental autoimmune encephalomyelitis.
Neuroscience. 132(4):1135-49. -
Penkowa, M. et al. (2003) Metallothionein-I overexpression alters brain inflammation and stimulates brain repair in transgenic mice with astrocyte-targeted interleukin-6 expression.
Glia. 42 (3): 287-306. -
Kim, S.R. et al. (2011) Dopaminergic pathway reconstruction by Akt/Rheb-induced axon regeneration.
Ann Neurol. 70: 110-20. -
Nordman, J.C. & Kabbani, N. (2012) An interaction between α7 nicotinic receptors and a G-protein pathway complex regulates neurite growth in neural cells.
J Cell Sci. 125 (Pt 22): 5502-13.
