Mouse anti Growth Cone antibody, clone 2G13
recognizes a protein originally termed 2G13P which is localized to growth cones. Subsequent investigation has identified this protein to be 40S ribosomal protein SA, also known as 37 kDa laminin receptor precursor or Laminin receptor 1 (Baloui et al. 2004
). 40S ribosomal protein SA is a 296 amino acid ~37 kDa membrane, cytoplasmic and nuclear protein required for the assembly and/or stability of the 40S ribosomal subunit.. In vertebrate evolution the molecule has acquired a secondary function as a laminin receptor (UniProt: P50890
). In growth cones expression is notable particularly in filopodia and lamellipodia in developing rat CNS and embryonic neurons in culture (Stettler et al. 1999
40S ribosomal protein SA interacts with the filamentous actin cytoskeleton and therefore may be involved in growth cone motility (Stettler et al. 1999
). Mouse anti Growth Cone antibody, clone 2G13 has been used for the detection of growth cones by immunohistochemistry and identification of 40S ribosomal protein SA by western blotting in chicken and rat samples (Baloui et al. 2004
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- Embryonic chick tectal membranes.
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
structural constituent of ribosome
small ribosomal subunit
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Growth Cone antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Clone 2G13 has also been reported to work in Western Blotting.
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
Stettler, O. et al. (1999) Monoclonal antibody 2G13, a new axonal growth cone marker.
J Neurocytol. 28 (12): 1035-44.
References for Growth Cone antibody
Baloui, H. et al. (2004) Cellular prion protein/laminin receptor: distribution in adult central nervous system and characterization of an isoform associated with a subtype of cortical neurons.
Eur J Neurosci. 20 (10): 2605-16.
Espejo, C. et al. (2005) Time-course expression of CNS inflammatory, neurodegenerative tissue repair markers and metallothioneins during experimental autoimmune encephalomyelitis.
Penkowa, M. et al. (2003) Metallothionein-I overexpression alters brain inflammation and stimulates brain repair in transgenic mice with astrocyte-targeted interleukin-6 expression.
Glia. 42 (3): 287-306.
Kim, S.R. et al. (2011) Dopaminergic pathway reconstruction by Akt/Rheb-induced axon regeneration.
Ann Neurol. 70: 110-20.
Nordman, J.C. & Kabbani, N. (2012) An interaction between α7 nicotinic receptors and a G-protein pathway complex regulates neurite growth in neural cells.
J Cell Sci. 125 (Pt 22): 5502-13.
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