Growth Cone antibody | 2G13

Mouse anti Growth Cone

Product Type
Monoclonal Antibody
Clone
2G13
Isotype
IgM
Specificity
Growth Cone

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MCA1712
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C P 2 ml loader
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Mouse anti Growth Cone antibody, clone 2G13 recognizes a protein originally termed 2G13P which is localized to growth cones. Subsequent investigation has identified this protein to be 40S ribosomal protein SA, also known as 37 kDa laminin receptor precursor or Laminin receptor 1 (Baloui et al. 2004). 40S ribosomal protein SA is a 296 amino acid ~37 kDa membrane, cytoplasmic and nuclear protein required for the assembly and/or stability of the 40S ribosomal subunit.. In vertebrate evolution the molecule has acquired a secondary function as a laminin receptor (UniProt: P50890). In growth cones expression is notable particularly in filopodia and lamellipodia in developing rat CNS and embryonic neurons in culture (Stettler et al. 1999).

40S ribosomal protein SA interacts with the filamentous actin cytoskeleton and therefore may be involved in growth cone motility (Stettler et al. 1999). Mouse anti Growth Cone antibody, clone 2G13 has been used for the detection of growth cones by immunohistochemistry and identification of 40S ribosomal protein SA by western blotting in chicken and rat samples (Baloui et al. 2004).

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Embryonic chick tectal membranes.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Clone 2G13 has also been reported to work in Western Blotting.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

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Source Reference

  1. Stettler, O. et al. (1999) Monoclonal antibody 2G13, a new axonal growth cone marker.
    J Neurocytol. 28 (12): 1035-44.

References for Growth Cone antibody

  1. Baloui, H. et al. (2004) Cellular prion protein/laminin receptor: distribution in adult central nervous system and characterization of an isoform associated with a subtype of cortical neurons.
    Eur J Neurosci. 20 (10): 2605-16.
  2. Espejo, C. et al. (2005) Time-course expression of CNS inflammatory, neurodegenerative tissue repair markers and metallothioneins during experimental autoimmune encephalomyelitis.
    Neuroscience. 132(4):1135-49.
  3. Penkowa, M. et al. (2003) Metallothionein-I overexpression alters brain inflammation and stimulates brain repair in transgenic mice with astrocyte-targeted interleukin-6 expression.
    Glia. 42 (3): 287-306.
  4. Kim, S.R. et al. (2011) Dopaminergic pathway reconstruction by Akt/Rheb-induced axon regeneration.
    Ann Neurol. 70: 110-20.
  5. Nordman, J.C. & Kabbani, N. (2012) An interaction between α7 nicotinic receptors and a G-protein pathway complex regulates neurite growth in neural cells.
    J Cell Sci. 125 (Pt 22): 5502-13.

Immunohistology - Frozen

Synonyms
40S Ribosomal Protein Sa
RRID
AB_322754
UniProt
P50890
Entrez Gene
RPSA
GO Terms
GO:0005886 plasma membrane
GO:0003735 structural constituent of ribosome
GO:0004872 receptor activity
GO:0005634 nucleus
GO:0006412 translation
GO:0015935 small ribosomal subunit
GO:0043236 laminin binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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