Alkaline Phosphatase antibody | AbD25296_hIgG2




Human anti Bacterial Alkaline Phosphatase:FITC
This FITC conjugated Anti-Bacterial Alkaline Phosphatase Antibody is recommended for use as a secondary detection reagent with HuCAL antibodies in Fab-A and Fab-Max formats.
Human anti Bacterial Alkaline Phosphatase:HRP
This HRP conjugated Anti-Bacterial Alkaline Phosphatase Antibody is recommended for use as a secondary detection reagent with HuCAL antibodies in Fab-A and Fab-Max formats.
- Product Type
- Monoclonal Antibody
- Clone
- AbD25296_hIgG2
- Isotype
- IgG2
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA275F | F | 0.1 mg |
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HCA275P | E WB | 0.1 mg |
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Fab-A and Fab-Max format antibodies generated using HuCAL® technology are formed via dimerization of BAP. The Anti-BAP Antibody HCA275P can be used as a secondary detection reagent with HuCAL antibodies in Fab-A and Fab-Max formats (Fab-Max is a modified BAP fusion antibody with inactivated enzymatic activity).
View further information about HuCAL antibody formats.
Product Details
- Target Species
- Bacterial
- Product Form
- Human IgG2 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Human IgG2 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Immunogen
- Bacterial alkaline phosphatase
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=38pM by real time, label-free molecular interaction analysis on immobilized HuCAL antibody in the Fab-A-FH format
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00634
- GO Terms
- GO:0005515 protein binding
- GO:0000287 magnesium ion binding
- GO:0004035 alkaline phosphatase activity
- GO:0008270 zinc ion binding
- GO:0030613 oxidoreductase activity, acting on phosphorus or arsenic in donors
- GO:0033748 hydrogenase (acceptor) activity
- GO:0042597 periplasmic space
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Regulatory
- For research purposes only
Applications of Alkaline Phosphatase antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/5 | 1/50 | |
ELISA | |||
Western Blotting | 1/1,000 | 1/10,000 |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Recombinant Human IgG2 Lambda | HCA108 | E | 0.1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
LYNX Rapid Fluorescein Antibody Conjugation Kit | LNK063F | CJ | 3 Conjugations For 20µg Antibody | ||
Mouse anti Human CD4:Alexa Fluor® 647 | MCA1267A647 | F | 100 Tests/1ml |
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Mouse anti Human CD3 | MCA2184 | F IP | 0.2 mg |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml |
Product Specific References
References for Alkaline Phosphatase antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-90.
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