READILINK™

ReadiLink Antibody Labeling Kits offer easy fluorescence conjugations for microscale volumes (50-100 µg). Each ReadiLink Dye is coupled to a reactive moiety (a succinimidyl ester). The reactive dye selectively binds to primary amines of proteins to form a stable carboxamide bond, ensuring no dissociation between fluorophore and antibody. After conjugation and the addition of the quencher buffer, any unbound ReadiLink Dye will bind to the quencher and become nonfluorescent.

The ReadiLink Antibody Labeling Kit provides all the essential components for performing two conjugation reactions (2 x 50 µg). Each kit can be used to label monoclonal or polyclonal antibodies or other proteins (>10 kDa).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.

Instructions For Use

Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.