READILINK™

100% Secure


READILINK™ 680/701 Antibody Labeling Kit

READILINK™ 405/454 Antibody Labeling Kit

READILINK™ 405/508 Antibody Labeling Kit

READILINK™ 405/537 Antibody Labeling Kit

READILINK™ 492/516 Antibody Labeling Kit

READILINK™ 555/570 Antibody Labeling Kit

READILINK™ 594/610 Antibody Labeling Kit

READILINK™ 633/655 Antibody Labeling Kit

READILINK™ 647/674 Antibody Labeling Kit

READILINK™ 700/713 Antibody Labeling Kit

READILINK™ 750/780 Antibody Labeling Kit

READILINK™ 790/811 Antibody Labeling Kit

Product Type
Conjugation Kit
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1351007 CJ 2 Conjugations For 50 µg Antibody
1351011 CJ 2 Conjugations For 50 µg Antibody
1351012 CJ 2 Conjugations For 50 µg Antibody
1351013 CJ 2 Conjugations For 50 µg Antibody
1351002 CJ 2 Conjugations For 50 µg Antibody
1351003 CJ 2 Conjugations For 50 µg Antibody
1351004 CJ 2 Conjugations For 50 µg Antibody
1351005 CJ 2 Conjugations For 50 µg Antibody
1351006 CJ 2 Conjugations For 50 µg Antibody
1351008 CJ 2 Conjugations For 50 µg Antibody
1351009 CJ 2 Conjugations For 50 µg Antibody
1351010 CJ 2 Conjugations For 50 µg Antibody
ReadiLink Antibody Labeling Kits offer easy fluorescence conjugations for microscale volumes (50-100 µg). Each ReadiLink Dye is coupled to a reactive moiety (a succinimidyl ester). The reactive dye selectively binds to primary amines of proteins to form a stable carboxamide bond, ensuring no dissociation between fluorophore and antibody. After conjugation and the addition of the quencher buffer, any unbound ReadiLink Dye will bind to the quencher and become nonfluorescent.

The ReadiLink Antibody Labeling Kit provides all the essential components for performing two conjugation reactions (2 x 50 µg). Each kit can be used to label monoclonal or polyclonal antibodies or other proteins (>10 kDa).

Product Details

Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer
Reagents in the Kit
2 vials ReadiLink Labeling Dye (powder) (Note: Use one vial to label 50 μg of antibodies)

1 vial (20 μl) Reaction Buffer

1 vial (20 μl) Quench Buffer

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.

More Information

Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Acknowledgements
ReadiLink™ is a trademark of AAT Bioquest, Inc.
Regulatory
For research purposes only

Applications of READILINK™

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Conjugation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.
Instructions For Use
Important: Thaw all kit components prior to use.

Note: The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.

Note: The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.

1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.

2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.

3. Mix well by pipetting up and down a few times.

4. Add the entire volume (55 μl) to one vial of labeling dye and mix by pipetting.

5. Incubate for 60 min at room temperature.

6. Add 5 μl quencher to the reaction mixture.

7. Incubate for 10 min at room temperature.

Antibodies are now labeled and ready to use.