PerCP-Cy5.5 Conjugation Kit



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F CJ ResetLYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit
- Product Type
- Conjugation Kit
- Specificity
- PerCP-Cy5.5 Conjugation Kit
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LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing Peridinin Chlorophyll protein (PerCP)-Cy5.5 label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of PerCP-Cy5.5 to the antibody. The LYNX Rapid Conjugation kits® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery. |
- Reagents In The Kit
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LNK144PERCPCY5.5: 3 Vials of 10ug LYNX lyophilized PerCP-Cy5.5 mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent -
LNK142PERCPCY5.5: 3 Vials of 100ug LYNX lyophilized PerCP-Cy5.5 mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent -
LNK141PERCPCY5.5: 1 Vial of 100ug LYNX lyophilized PerCP-Cy5.5 mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent - Preparing The Antibody
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LNK144PERCPCY5.5
The following buffer solutions are recommended for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
The molar ratio of antibody: PerCP-Cy5.5 should be 1:1, i.e. 10ug antibody to every 10ug PerCP-Cy5.5. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 10ul and a maximum antibody amount of 10ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit. -
LNK142PERCPCY5.5, LNK141PERCPCY5.5
The following buffer solutions are recommended for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
The molar ratio of antibody: PerCP-Cy5.5 should be 1:1, i.e. 100ug antibody to every 100ug PerCP-Cy5.5. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 100ul and a maximum antibody amount of 100ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit. - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
- Licensed Use
- These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: antibodies@bio-rad.com
Application Name | Verified | Min Dilution | Max Dilution |
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Conjugation | ![]() |
We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
- Instructions For Use
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LNK144PERCPCY5.5, LNK142PERCPCY5.5, LNK141PERCPCY5.5
1.To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.
2.Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.
3.Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.
4.After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
References for PerCP-Cy5.5 Conjugation Kit
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Jamaly, S. et al. (2018) Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism.
J Thromb Haemost. May 31 [Epub ahead of print]. -
Jax, E. et al. (2023) Evaluating Effects of AIV Infection Status on Ducks Using a Flow Cytometry-Based Differential Blood Count.
Microbiol Spectr. : e0435122. -
Sabsabi, M.A. et al. (2024) Unraveling the role of γδ T cells in the pathogenesis of an oncogenic avian herpesvirus.
mBio. 15 (8): e0031524. -
Chi, Z. et al. (2024) snRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle.
BMC Genomics. 25 (1): 999.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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