Endotoxin Removal Standard Kit

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Proteus MIDI Endotoxin Removal Standard Spin Column Pack

Product Type: Purification Kit

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PUR028	PP			12 Units

Summary
References (1)

This kit offers rapid, one step, high grade (>95%) removal of endotoxin from a wide range of protein solutions, with >90% protein recovery.

We recommend reading the Proteus Endotoxin Removal Kit Handbook prior to use.

Product Details

Preservative Stabilisers: 94% Preservative base of resin containing:
20% Aqueous Ethanol
80% 0.1M Sodium Chloride
Reagents in the Kit: 12 x Spin Column Plugs containing Endotoxin Binding Resin
12 x Midi Standard Spin Columns (20ml capacity)
12 x 50ml Centrifuge Tubes
Plug Insertion Tool
Instructions for use
Endotoxin Level: This kit can be used for samples with endotoxin loads < 30,000 EU. For endotoxin loads < 3,000 EU the Mini Endotoxin Removal kit is recommended. Endotoxin loads up to 1,000,000 EU require the use of a Midi High Capacity Endotoxin Removal kit.
Additional Reagents Required: 0.2µm and 1.2µm Syringe Filter Units
Low Endotoxin Pre-equilibration Buffer e.g PBS pH7.4

Storage Information

Storage: Upon receipt of kit, remove resin plugs box and store this at +4^oC.
DO NOT FREEZE RESIN PLUGS OR STORE AT ROOM TEMPERATURE.
Store remainder of kit at room temperature.
Shelf Life: Please see label for expiry date.

More Information

Regulatory: For research purposes only

Applications of Endotoxin Removal Standard Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Protein Purification

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

Instructions For Use: Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/PUR028.pdf

Product Specific References

References for Endotoxin Removal Standard Kit

Negus, D. et al. (2015) Parenteral Administration of Capsule Depolymerase EnvD Prevents Lethal Inhalation Anthrax Infection.
Antimicrob Agents Chemother. pii: AAC.01547-15.

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