IFN Gamma

100% Secure


Bovine Interferon Gamma ELISA Kit

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5638KZZ E 5 X 96 Wells
The Bovine Interferon Gamma Kit is specific for bovine interferon gamma (IFN-gamma), a secreted cytokine involved in anti-viral activity and growth regulation belonging to the type II interferon family.

IFN-gamma is produced by lymphocytes in response to activation by specific antigens or mitogens. In addition to its anti-viral activity, IFN-gamma has important immunoregulatory functions in the activation of macrophages and anti-proliferative effects upon transformed cells.

Product Details

Intended Use
Bovine Interferon gamma ELISA KIt (MCA5638KZZ) is an ELISA kit (5 x 96 wells) designed for the quantitive determination of bovine interferon gamma (BoIFN-gamma) in bovine serum, plasma and culture supernatant.

This assay can measure BoIFN-gamma in the range 0.025-50 ng/ml in bovine serum, plasma and culture supernatant. Detailed instructions for use of the kit can be found here.
Target Species
Bovine
Reagents in the Kit
96 well microtiter plates x 5
Microplate adhesive sealers x 5
Coating antibody - 1vial (MCA5638KZZA)
Coating Buffer - 1 vial (5 x concentrated)
Detection antibody – Biotin conjugated - 1 vial (MCA5638KZZC)
Streptavidin:HRP conjugate - 1 vial (MCA5638KZZD)
TMB Substrate 1 vial
Bovine IFN gamma standard (400ng/ml – Lyophilized) 1 vial (MCA5638KZZB)
Test Principle
This assay is based on a sandwich ELISA using two different mouse anti bovine IFN-gamma monoclonal antibodies and a recombinant bovine IFN-gamma as a standard.
Additional Reagents Required
The following additional reagents and Equipment are also required for successful use of this kits BUT ARE NOT CONTAINED WITHIN THIS KIT

Wash buffer (available from Bio-Rad, BUF031A, or see kit insert for recipe)
Blocking solution (available from Bio-Rad, Catalogue Code: BUF032A, or see kit insert for recipe)
Stop Solution (see kit insert for recipe)
Distilled or Deionised water
Plate reader with 450nm reading capacity
Pipettes for dispensing up to 250ul
Tubes for preparing standard and sample dilutions.

Storage Information

Storage
Prior to use store all kit components at +4oC. DO NOT FREEZE. Please view the handbook for further details.
Storage in frost free freezers is not recommended. This Kit contains components that are photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature components within this kit.
The contents of this kit should be stored undiluted. Should components within this kit contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Stored correctly, 18 months from date of despatch.

More Information

UniProt
P07353 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
This kit and its components are Research Use Only. Not for Therapeutic or Diagnostic use.

Applications of IFN Gamma

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/MCA5638KZZ.pdf

Product Specific References

References for IFN Gamma

  1. Doherty, R. et al. (2013) Epigenetic regulation of the innate immune response to LPS in bovine peripheral blood mononuclear cells (PBMC)
    Vet Immunol Immunopathol. Aug 15;154: 102-10.
  2. Redondo, E. et al. (2014) Induction of Interleukin-8 and Interleukin-12 in Neonatal Ovine Lung Following Experimental Inoculation of Bovine Respiratory Syncytial Virus.
    J Comp Pathol. 150: 434-48.
  3. Truong, T. et al. (2014) Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection.
    PLoS One. 9: e87145.
  4. Blodörn, K. et al. (2015) A bovine respiratory syncytial virus model with high clinical expression in calves with specific passive immunity.
    BMC Veterinary Research. 11 (1): 76.
  5. Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
    PLoS One. 9 (6): e100392.
  6. Beechler, B.R. et al. (2017) Host immunity, nutrition and coinfection alter longitudinal infection patterns of schistosomes in a free ranging African buffalo population.
    PLoS Negl Trop Dis. 11 (12): e0006122.
  7. Shaw, A.E. et al. (2017) Fundamental properties of the mammalian innate immune system revealed by multispecies comparison of type I interferon responses.
    PLoS Biol. 15 (12): e2004086.
  8. TakeetMichael, I. et al. (2016) Differential IFN-Gamma (IFN-γ), Interleukin 10 (IL-10) and Cardiac Troponin I (cTnI) Responses in Natural Bovine Trypanosomosis in Nigeria
    Open Journal of Veterinary Medicine. 06 (07): 105-111.