IgM Antibody

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  • Goat anti Human IgM:Biotin
  • Goat anti Human IgM:Texas Red®
  • Goat anti Human IgM:Tritc
  • Goat anti Human IgM:Alk. Phos.
  • Goat anti Human IgM:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    202009Fdatasheet pdfdatasheet pdf0.5 mg
    202009
    202008E, F, IBdatasheet pdfdatasheet pdf1 mg
    202008
    202007Fdatasheet pdfdatasheet pdf1 mg
    202007
    202003C, Fdatasheet pdfdatasheet pdf1 mg
    202003
    202004C, E, IBdatasheet pdfdatasheet pdf1 ml
    202004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti human IgM polyclonal antibody reacts with the heavy chain of human IgM. This antibody has been cross-absorbed to reduce cross-reactivity with human IgG and IgA, but may react with IgM from other species.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Texas Red® (TXRD) - Liquid
      Purified IgG conjugated to Rhodamine (TRITC) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human IgM was raised by repeated immunisation of goats with human IgM paraproteins. The preparation was purified by affinity chromatography on human IgM covalently linked to Sepharose-4B.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1% Sodium Azide (NaN3)
      0.1% Sodium Azide (NaN3)
      0.1% Sodium Azide
      50% Glycerol
      0.1% Sodium Azide
      Stabilising agent
    • Immunogen
      Human IgM
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      Ig concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      TRIS buffered saline, 1mM MgCl2
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from the date of despatch.
      Please see label for expiry date.
      18 months from the date of despatch.
      Please see label for expiry date.
      Please see label for expiry date.
    • GO Terms
      integral to membrane
      antigen binding
      membrane fraction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5,0001/20,000
      Flow CytometryNeat1/10
      Immunoblotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
      Immunoblotting
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgM Antibody Formats

    Formats Applications Sizes available
    IgM Antibody : RPE F 0.5 mg
    IgM Antibody : Tritc C, F 1 mg
    IgM Antibody : Biotin E, F, IB 1 mg
    IgM Antibody : Texas Red® F 1 mg
    IgM Antibody : Alk. Phos. C, E, IB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

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