HEXIM1 Antibody (PrecisionAbTM Antibody)

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HEXIM1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with HEXIM1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 32 second exposure. Arrow points to HEXIM1 (molecular weight 70 kDa)

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  • HEXIM1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • HEXIM1 Antibody with Control Lysate
  • Goat anti HEXIM1
  • HEXIM1 Antibody with Control Lysate
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    HEXIM1 antibody recognizes protein HEXIM1, also known as cardiac lineage protein 1, estrogen down-regulated gene 1 protein, hexamethylene bis-acetamide-inducible protein 1 or menage a quatre protein 1. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00125WBdatasheet pdfdatasheet pdf100 µl
      VPA00125
      VPA00125KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00125K
      VPA00125KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00125KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human HEXIM1 antibody recognizes protein HEXIM1, also known as cardiac lineage protein 1, estrogen down-regulated gene 1 protein, hexamethylene bis-acetamide-inducible protein 1 or menage a quatre protein 1.

        Expression of HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. HEXIM1 has no introns (provided by RefSeq, Jul 2008).

        Goat anti Human HEXIM1 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti HEXIM1 detects a band of approximately 70 kDa in K562 cell lysates.
        Anti HEXIM1 detects a band of approximately 70 kDa in K562 cell lysates.
        Anti HEXIM1 detects a band of approximately 70 kDa in K562 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Goat polyclonal antibody purified by affinity chromatography.
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
      • Immunogen
        Peptide with the sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer.
        400μg K562 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        transcription
        cyclin-dependent protein kinase inhibitor activity
        negative regulation of cyclin-dependent protein kinase activity
        cytoplasm
        nucleus
        protein binding
        snRNA binding
        negative regulation of transcription from RNA polymerase II promoter
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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