CD52 Antibody | YTH66.9HL

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  • Product Type
    Monoclonal Antibody
  • Clone
    YTH66.9HL
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA349RC, CT, F, WBdatasheet pdfdatasheet pdf0.1 ml
    MCA349R
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Human CD52 antibody, clone YTH66.9HL recognizes human CD52, also known as CAMPATH-1. The CD52 antigen is a small heavily glycosylated peptide attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kDa.

      CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

      Humanised versions of CAMPATH-1 specific antibodies are used for the treatment of a range of lymphoid malignancies (Blatt et al. 2014).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Cynomolgus monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ig fraction - liquid
    • Reconstitution
    • Preparation
      Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Human T lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag.1.2.3 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      elevation of cytosolic calcium ion concentration
      respiratory burst
      integral to plasma membrane
      anchored to membrane
      membrane fraction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Cytotoxic Assays
      Flow Cytometry1/20
      Immunohistology - Frozen1/20
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD52 Antibody Formats

    Formats Clone Applications Sizes available
    CD52 Antibody : Purified YTH66.9HL C, CT, F, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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