MCA792F recognises a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia.
The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al. has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.
0.1% Sodium Azide (NaN3) 0.2% Bovine Serum Albumin
HRIK cells - Human B-Lymphoblastoid line
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
TRIS buffered glycine
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
1. Brooks D.A., et al. (1981) Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. IV. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes.J. Immunol. 126: 1373.
2. Catovsky, D. et al. (1981) Heterogeneity of B-cell leukemias demonstrated by the monoclonal antibody FMC7.Blood 58: 406 - 408
4. Zola H., et al. (1984) The antigen of mature human B cells detected by the monoclonal antibody FMC7: studies on the nature of the antigen and modulation of its expression.J. Immunol. 133: 321-326.
5. Bloem A.C., et al. (1988) Functional properties of human B cell populations defined by monoclonal antibodies HBA and FMC7.J. Immunol. 140: 768-773.
6. Zola H., et al. (1987) Markers of differentiated B cell leukaemia: CD22 antibodies and FMC7 react with different molecules.Dis. Markers 5: 227-235.
7. Melo I.V., et al. (1988) The different diagnosis between chronic lymphocytic leukemia and other B-cell lymphoproliferative disorders: morphological and immunological studies. In: Chronic Lymphocytic Leukemia (eds. A. Polliack and D. Catovsky) p85.Harwood acad. Publ. London.
8. Ferro, L.M. and Zola H. (1990) Modulation of expression of the antigen identified by FMC7 upon human B-lymphocyte activation: evidence for differences between activation in vivo and in vitroImmunology. 69: 373-378.
9. Collins R.J., et al. (1992) Malignant lymphoma: reactive with the monoclonal antibody FMC7.Pathology 15: 350.
10. Serke, S. et al. (2001) Monoclonal antibody FMC7 detects a conformational epitope on the CD20 molecule:Evidence from phenotyping after Rituxan therapy and transfectant cell analyses.Cytometry (Comm. Clin. Cytometry) 46:98-104