IgG Antibody

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  • Rabbit F(ab')2 anti Goat IgG (H/L):Alk. Phos.
  • Rabbit F(ab')2 anti Goat IgG (H/L):FITC
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    602002E, Fdatasheet pdfdatasheet pdf0.5 mg
    602002
    602004C, E, IB, WBdatasheet pdfdatasheet pdf1 ml
    602004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit F(ab')2 anti goat IgG (H/L) is specific for the heavy and light chains of goat IgG as demonstrated by ELISA. It may also react with the light chains of other goat immunoglobulins.
    • Intended Use
    • Target Species
      Goat
    • Product Form
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to Goat IgG (H&L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were generated by pepsin digestion.
    • Preservative Stabilisers
      0.1% Sodium azide
      50% Glycerol
      0.1% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline, 1mM MgCl2
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Please see label for expiry date.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2,0001/4,000
      Immunoblotting
      Immunohistology - Frozen
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2001/400
      Flow CytometryNeat1/5

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : FITC E, F 0.5 mg
    IgG Antibody : Alk. Phos. C, E, IB, WB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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