100% secure
Email this product page
Print this page

Aspergillus Antibody | WF-AF-1

Aspergillus Antibody | WF-AF-1 gallery image 1

Human Placental tissue presenting aspergillosis stained with Mouse anti Aspergillus antibody, clone WF-AF-1 (MCA2576)

  • Aspergillus Antibody | WF-AF-1 thumbnail image 1
  • Mouse anti Aspergillus SPP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2576E, IP, P *, WBdatasheet pdfdatasheet pdf0.25 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Aspergillus spp., clone WF-AF-1, is raised against the wall fraction (WF) of Aspergillus fumigatus. This antibody specifically recognises members of the Aspergillus spp. including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of hyphae.

      A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. Clone WF-AF-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. Clone WF-AF-1 has also been used for the identification of aspergillosis in human tissue sections.

      Clone WF-AF-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ammonium sulfate precipitation from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Wall fraction (WF) of Aspergillus fumigatus
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunohistology - Paraffin(1)1/300
      This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Aspergillus infected placenta.
    • Immunofluorescence
    • Western Blotting
      Mouse anti Aspergillus spp. antibody, clone WF-AF-1 detects a band of approximately 106kDa of Aspergillus fumigatus wall fraction (WF).
    • Instructions For Use

    Additional Aspergillus Antibody Formats

    Formats Clone Applications Sizes available
    Aspergillus Antibody : Purified WF-AF-1 E, IP, P *, WB 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      Goat anti Mouse IgM:FITCSTAR86F1 mgF
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Aspergillus infected placenta.
                • Product Images

                • Aspergillus Antibody | WF-AF-1 thumbnail image 1
              • Application NameReference Images
                Immunohistology - Paraffin


              Write your review

              You may also be interested in...