Aspergillus Antibody | WF-AF-1

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Aspergillus Antibody | WF-AF-1 gallery image 1

Human Placental tissue presenting aspergillosis stained with Mouse anti Aspergillus antibody, clone WF-AF-1 (MCA2576)

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  • Mouse anti Aspergillus SPP
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  • Product Type
    Monoclonal Antibody
  • Clone
    WF-AF-1
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2576E, IP, P *, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA2576
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Aspergillus spp., clone WF-AF-1, is raised against the wall fraction (WF) of Aspergillus fumigatus. This antibody specifically recognises members of the Aspergillus spp. including A. flavus and A. niger, reacting strongly with walls and septae, and to a lesser extent within the cytoplasm of hyphae.

      A. fumigatus, a thermophilic, opportunistic and angio-invasive filamentous fungus, is the main causative agent of systemic bovine aspergillosis, a worldwide and often fatal respiratory disease of cattle. Clone WF-AF-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of bovine systemic aspergillosis, attributed to its binding to the major cell wall component, galactomannan. Clone WF-AF-1 has also been used for the identification of aspergillosis in human tissue sections.

      Clone WF-AF-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp., but may react with galactomannans of members of the genus Penicillium.
    • Intended Use
    • Target Species
      Fungal
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ammonium sulfate precipitation from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Wall fraction (WF) of Aspergillus fumigatus
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)1/300
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Aspergillus infected placenta.
    • Immunofluorescence
    • Western Blotting
      Mouse anti Aspergillus spp. antibody, clone WF-AF-1 detects a band of approximately 106kDa of Aspergillus fumigatus wall fraction (WF).
    • Instructions For Use

    Additional Aspergillus Antibody Formats

    Formats Clone Applications Sizes available
    Aspergillus Antibody : Purified WF-AF-1 E, IP, P *, WB 0.25 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Aspergillus infected placenta.
              • Application NameReference Images
                Immunohistology - Paraffin

              References

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