Browsing Modes for Business Users
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Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
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Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and will not have access to any other carts
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Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Browsing Modes for Business Users
-
Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
-
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and will not have access to any other carts
-
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Browsing Modes for Business Users
-
Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
-
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and will not have access to any other carts
-
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Flow Cytometry Guide: Intracellular Antigens
Overview
Staining intracellular antigens like cytokines can be difficult because antibody-based probes cannot pass easily through the plasma membrane into the interior of the cell. In order to accomplish this, cells should first be fixed in suspension and then permeabilized before adding the fluorophore. This allows probes to access intracellular structures while leaving the morphological scatter characteristics of the cells intact. Octylphenol ethoxylate is one example of a permeabilization reagent (Figure 18). There are also many commercial kits available today that provide the reagents to carry out these crucial steps, for example, Leucoperm™.
Fig. 18. 0.1% octylphenol ethoxylate used in conjunction with phalloidin, which recognizes and binds to filamentous actin. A, before treatment of cells; B, after fixation and permeabilization. Notice how distinctive the positive dataset becomes. FL, fluorescence.
Popular Resources
Blog: Sample Preparation in Flow Cytometry — Tips & Tricks
In this blog, we provide tips and advice on generating a high-quality sample for flow cytometry.
Quick Guide: How to Implement an Effective Flow Cytometry Gating Strategy
Download this quick guide to learn how to develop effective flow cytometry gating strategies, avoid common pitfalls such as false positives and missed rare populations, and generate reliable, reproducible data through a systematic approach to panel design and analysis.