Ig Antibody | 5F12

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Ig Antibody | 5F12 gallery image 1

Published customer image:
Mouse anti Salmonid Ig antibody, clone 5F12 used for the detection of rainbow trout IgM by ELISA
Image caption:
Detection of Y. ruckeri specific IgM antibodies in plasma post ERM immersion vaccination. The ELISA method was used to detect Y. ruckeri specific IgM antibodies in plasma. The antibody titer were significantly higher in immersion vaccinated groups compared to the sham vaccinated group (*P<0.05).

From: Raida MK, Nylén J, Holten-Andersen L, Buchmann K (2011) Association between Plasma Antibody Response and Protection in Rainbow Trout Oncorhynchus mykiss Immersion Vaccinated against Yersinia ruckeri. PLoS ONE 6(6): e18832.

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Ig Antibody | 5F12 gallery image 2

Published customer image:
Mouse anti Salmonid Ig antibody, clone 5F12 used for the detection of rainbow trout immunoglobulin by ELISA
Image caption:
Antibody measurements and parasite load after challenge from T2. The graph shows both the measurement by OD(450) of I. multifiliis specific antibodies and the parasite burden of O. mykiss following challenge with I. multifiliis after DNA vaccination with pcDNA3.1-CassN-Iag52B and pcDNA3.1-CassN-ICP2. Challenge was conducted 47 dpv. * indicates a statistical difference between the empty vector group and both the control group and the group injected with the combination subepidermally.

From: von Gersdorff Jørgensen L, Sigh J, Kania PW, Holten-Andersen L, Buchmann K, et al. (2012) Approaches towards DNA Vaccination against a Skin Ciliate Parasite in Fish. PLoS ONE 7(11): e48129.

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Ig Antibody | 5F12 gallery image 3

Published customer image:
Mouse anti Salmonid Ig antibody, clone 5F12 used for the detection of rainbow trout immunoglobulin by ELISA
Image caption:
Antibody measurements and parasite load after challenge from T3. The graph shows both the measurement by OD(450) of I. multifiliis specific antibodies and the parasite burden of O. mykiss following challenge with I. multifiliis after DNA vaccination with pVax-CassL-Iag52B-EEF and pcDNA3.1-CassN-ICP2. Challenge was conducted 80 dpv. No statistical differences were observed between the groups.

From: von Gersdorff Jørgensen L, Sigh J, Kania PW, Holten-Andersen L, Buchmann K, et al. (2012) Approaches towards DNA Vaccination against a Skin Ciliate Parasite in Fish. PLoS ONE 7(11): e48129.

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Ig Antibody | 5F12 gallery image 4

Published customer image:
Mouse anti Salmonid Ig antibody, clone 5F12 used for the detection of rainbow trout Ig by ELISA
Image caption:
Specific antibody response. Figures 2A–F show the results of the ELISA performed on sera collected from each experimental group at each of the 7 sampling time points. Empty diamond: Pre vaccination Filled square: control, filled diamond: experimental vaccine, Filled circle: Freunds incomplete adjuvant, Filled upward facing triangle: AlphaJect 3000, Filled downward facing triangle: PHARMAQ adjuvant. ANOVA analysis of ELISA results were performed on the measured absorbance of the separate 1:25 dilutions in each group. Asterisks indicate statistically significant difference between treated group and infected controls (* = P<0.05, ** = P<0.01, *** = P<0.001). All data shown are mean absorbance ± SEM.

From: Rømer Villumsen K, Dalsgaard I, Holten-Andersen L, Raida MK (2012) Potential Role of Specific Antibodies as Important Vaccine Induced Protective Mechanism against Aeromonas salmonicida in Rainbow Trout. PLoS ONE 7(10): e46733.

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Ig Antibody | 5F12 gallery image 5

Published customer image:
Mouse anti Salmonid Ig antibody, clone 5F12 used for the detection of rainbow trout Ig by ELISA
Image caption:
Antibody titers. Mean calculated titers ± SEM for each group at each time point. The titers were calculated as described in the main text. Differences in antibody titer between control and treated groups were analyzed using the Kruskal-Wallis method. Asterisks indicate statistically significant difference between treated group and infected controls (* = P<0.05, ** = P<0.01). See legend for identification of the experimental groups.

From: Rømer Villumsen K, Dalsgaard I, Holten-Andersen L, Raida MK (2012) Potential Role of Specific Antibodies as Important Vaccine Induced Protective Mechanism against Aeromonas salmonicida in Rainbow Trout. PLoS ONE 7(10): e46733.

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Ig Antibody | 5F12 gallery image 6

Published customer image:
Mouse anti Salmonid Ig antibody, clone 5F12 used for the detection of rainbow trout Ig by ELISA
Image caption:
Detection of Y. ruckeri specific antibodies in plasma.Plasma samples were collected A) Pre-booster vaccination B) Pre-challenge infection and C) seven days post infection. No statistical significances in the antibody levels were found among all groups at any of the sampling time points.

From: Villumsen KR, Neumann L, Ohtani M, Strøm HK, Raida MK (2014) Oral and Anal Vaccination Confers Full Protection against Enteric Redmouth Disease (ERM) in Rainbow Trout. PLoS ONE 9(4): e93845.

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  • Mouse anti Salmonid Ig
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5F12
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2182E, F, IF, WBdatasheet pdfdatasheet pdf0.5 ml
    MCA2182
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Salmonid Ig monoclonal antibody, clone 5F12 recognizes salmonid immunoglobulin heavy chain, binding to Ig of the Salmo, Oncorhynchus and Salvelinus genera. These include:

      Salmo:
      Atlantic Salmon
      Brown Trout

      Oncorhynchus:
      Chinook Salmon
      Chum Salmon
      Coho Salmon
      Sockeye Salmon
      Rainbow Trout

      Salvelinus:
      Arctic Char
      Brook Trout

      This antibody does not detect immunoglobulin from other fish such as China Perch or Zebra Fish.

      The antibody may be used to detect increased levels of immunoglobulins in fish serum, which may be indicative of a parasitic or viral infection.
    • Intended Use
    • Target Species
      Fish
    • Product Form
      Tissue Culture Supernatant - liquid (concentrated)
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Rainbow trout and Atlantic salmon immunoglobulins.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2501/1000
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunohistology - Resin
      Western Blotting1/2501/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      This antibody detects a band of approximately 71 kDa using Salmon immune serum.
    • Instructions For Use

    Additional Ig Antibody Formats

    Formats Clone Applications Sizes available
    Ig Antibody : Con S/N 5F12 E, F, IF, WB 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Rainbow Trout IgMCA59760.5 mlC, E, IF, WB
          MCA5976
          Mouse anti IsavMCA59770.5 mlC, E, IF
          MCA5977
          Mouse anti Flavobacterium Psychrophilum:FITCMCA5978F0.1 mgIF
          MCA5978F
          Mouse anti Flavobacterium Psychrophilum:HRPMCA5978P0.1 mgE, WB
          MCA5978P
          Mouse anti Kudoa thyrsitesMCA59790.25 mgE, F, IF, WB
          MCA5979
          Rabbit anti Salmonid IgAHP76150 µlE, IF, WB
          AHP761

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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