CD4/CD8 | YKIX302.9/YCATE55.9

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CD4/CD8 | YKIX302.9/YCATE55.9 gallery image 1

FITC conjugated Rat anti Canine CD4 and PE conjugated Rat anti Canine CD8 (DC048). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat anti Dog CD4:FITC/CD8:RPE
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  • Product Type
    Dual Color Reagent
  • Clone
    YKIX302.9/YCATE55.9
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    DC048Fdatasheet pdfdatasheet pdf100 Tests
    DC048
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Canine CD4/CD8 antibodies, clones YKIX302.9 and YCATE55.9 is a dual colour reagent recognising canine CD4 and CD8 cell surface antigens. CloneYKIX302.9 recognises canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.

      Clone YCATE55.9 recognises CD8α expressed by cytotoxic T cells.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      FITC reagent: IgG2a (RAT)
      RPE reagent: IgG1 (RAT)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD4/CD8 Formats

    Formats Clone Applications Sizes available
    CD4/CD8 : FITC/RPE YKIX302.9/YCATE55.9 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a:FITC/Rat IgG1:RPE Negative ControlDC05250 TestsF
        DC052

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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