Browsing Modes for Business Users

Rat IgG2b Negative Control antibody

ISO 9001 Quality Assurance Manufacturing | Print
Rat IgG2b Negative Control Antibody thumbnail image 1 Rat IgG2b Negative Control Antibody thumbnail image 2 Rat IgG2b Negative Control Antibody thumbnail image 3
Rat IgG2b Negative Control Antibody gallery image 1
Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat IgG2b isotype control (MCA6006F). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat anti Human CD18 (MCA503F). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Rat IgG2b Negative Control Antibody gallery image 2
Figure A. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat IgG2b isotype control (MCA6006PE). Figure B. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat anti Human CD28 (MCA709PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Rat IgG2b Negative Control Antibody gallery image 3
Figure A. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control (MCA6006F). Figure B. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat anti Canine CD4 FITC (MCA1038F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Filter by Application:

F

Rat IgG2b Negative Control:Alexa Fluor® 700

Product Type
Negative/Isotype Control
Isotype
IgG2b
Specificity
Rat IgG2b Negative Control

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA6006A700
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader

Rat IgG2b negative control, a rat monoclonal raised against KLH, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2b test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.		 Isotype controls savings up to 65%

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®700 702 723
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for Rat IgG2b Negative Control antibody

  1. Stavre, Z. et al. (2022) A role for neutrophils in early enthesitis in spondyloarthritis.
    Arthritis Res Ther. 24 (1): 24.
  2. Andrews, S.L. et al. (2023) SVEP1 influences monocyte to macrophage differentiation via integrin α4β1/α9β1 and Rho/Rac signalling.
    Biochim Biophys Acta Mol Cell Res. 1870 (6): 119479.

MCA6006A700

1702

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Negative Control Products
Please note