APC Conjugation Kit

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Published customer image:
LYNX Rapid APC Antibody Conjugation Kit used for the conjugation of anti VAP-1 antibody to allophycocyanin for subsequent detection of VAP-1 in colon tissues by immunofluorescence.
Image caption:
Dual-colour immunofluorescent staining of sections of normal colon tissue and cultured colonic endothelial cells. Magnification x630. a VAP-1 staining is present in SMA+ cells of vessels in sections of colonic mucosa. b VAP-1 staining does not co-localise with CD31+ endothelial staining of vessels in sections of colonic mucosa. Right-most images are digitally magnified x3. Yellow staining represents autofluorescence from erythrocytes. c Positive CD31 staining (left) and absent VAP-1 staining (right) of cultured colonic endothelial cells

From: Ward ST, Weston CJ, Shepherd EL, Hejmadi R, Ismail T, Adams DH.
Evaluation of serum and tissue levels of VAP-1 in colorectal cancer.
BMC Cancer. 2016 Feb 24;16(1):154.

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  • APC Conjugation Kit thumbnail image 1
  • LYNX Rapid APC Antibody Conjugation Kit
  • LYNX Rapid APC Antibody Conjugation Kit
  • LYNX Rapid APC Antibody Conjugation Kit
  • LYNX Rapid APC Antibody Conjugation Kit
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  • Product Type
    Conjugation Kit
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    LNK033APCCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf1 Conjugation For 1.5mg Antibody
    LNK033APC
    LNK031APCCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf1 Conjugation For 150µg Antibody
    LNK031APC
    LNK034APCCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf3 Conjugations For 10µg Antibody
    LNK034APC
    LNK032APCCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf3 Conjugations For 150µg Antibody
    LNK032APC
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • LYNX Rapid APC Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing Allophycocyanin (APC) label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of APC to the antibody.

      The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 150µg Antibody1 Vial of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 150µg Antibody3 Vials of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 1.5mg Antibody1 Vial of 1mg LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 150µg Antibody1 Vial of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 150µg Antibody3 Vials of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 1.5mg Antibody1 Vial of 1mg LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 150µg Antibody1 Vial of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 150µg Antibody3 Vials of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 1.5mg Antibody1 Vial of 1mg LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 150µg Antibody1 Vial of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 150µg Antibody3 Vials of 100ug LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 1.5mg Antibody1 Vial of 1mg LYNX lyophilized APC mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
    • Preparing The Antibody
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 10-15ug of antibody be used for every 10ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 4-10ul.
      Pack Size: 1 Conjugation For 150µg Antibody, 3 Conjugations For 150µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 40ul although up to 100ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 1.5mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 400ul although up to 1000ul is acceptable where the antibody concentration is low.
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 10-15ug of antibody be used for every 10ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 4-10ul.
      Pack Size: 1 Conjugation For 150µg Antibody, 3 Conjugations For 150µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 40ul although up to 100ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 1.5mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 400ul although up to 1000ul is acceptable where the antibody concentration is low.
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 10-15ug of antibody be used for every 10ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 4-10ul.
      Pack Size: 1 Conjugation For 150µg Antibody, 3 Conjugations For 150µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 40ul although up to 100ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 1.5mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 400ul although up to 1000ul is acceptable where the antibody concentration is low.
      Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 10-15ug of antibody be used for every 10ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 4-10ul.
      Pack Size: 1 Conjugation For 150µg Antibody, 3 Conjugations For 150µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 40ul although up to 100ul is acceptable where the antibody concentration is low.
      Pack Size: 1 Conjugation For 1.5mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-150ug of antibody be used for every 100ug APC, corresponding to a 1:1 antibody: APC molar ratio. For optimal results the antibody volume should be around 400ul although up to 1000ul is acceptable where the antibody concentration is low.
    • Test Principle
    • Buffer Solution
    • Storage
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
    • Shelf Life
      12 months after despatch
      12 months after despatch
      12 months after despatch
      12 months after despatch
    • Acknowledgements
    • Licensed Use
      These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: antibodies@bio-rad.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation


    • We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    Additional APC Conjugation Kit Formats

    Formats Applications Sizes available
    APC Conjugation Kit : Kit CJ 3 Conjugations For 10µg Antibody | 1 Conjugation For 150µg Antibody | 3 Conjugations For 150µg Antibody | 1 Conjugation For 1.5mg Antibody
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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